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Anti-ABCE1 antibody [EPR15373(B)] - C-terminal (ab185548)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15373(B)] to ABCE1 - C-terminal
  • Suitable for: ICC/IF, IP, WB, IHC-P, Flow Cyt
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-ABCE1 antibody [EPR15373(B)] - C-terminal
    See all ABCE1 primary antibodies

  • Description

    Rabbit monoclonal [EPR15373(B)] to ABCE1 - C-terminal

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Mouse
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • 293, K562 and HeLa cell lysate. Human breast carcinoma. Mouse liver. HeLa and 293 cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-ABCE1 antibody [EPR15373(B)] - C-terminal (ab185548)
    Western blot - Anti-ABCE1 antibody [EPR15373(B)] - C-terminal (ab185548)
    All lanes : Anti-ABCE1 antibody [EPR15373(B)] - C-terminal (ab185548) at 1/5000 dilution

    Lane 1 : 293 cell lysate
    Lane 2 : K562 cell lysate
    Lane 3 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 67 kDa
    Observed band size: 67 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCE1 antibody [EPR15373(B)] - C-terminal (ab185548)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCE1 antibody [EPR15373(B)] - C-terminal (ab185548)

    Immunohistochemical analysis of paraffin embedded Human breast carcinoma tissue sections labeling ABCE1 using ab185548 at a 1/100 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCE1 antibody [EPR15373(B)] - C-terminal (ab185548)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCE1 antibody [EPR15373(B)] - C-terminal (ab185548)

    Immunohistochemical analysis of paraffin embedded mouse liver tissue sections labeling ABCE1 using ab185548 at a 1/100 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-ABCE1 antibody [EPR15373(B)] - C-terminal (ab185548)
    Immunocytochemistry/ Immunofluorescence - Anti-ABCE1 antibody [EPR15373(B)] - C-terminal (ab185548)

    Immunofluorescent analysis of formaldehyde fixed HeLa cells labeling ABCE1 using ab185548 at a 1/250 dilution. A Goat anti rabbit IgG (Alexa Fluor®555) was used as the secondary at a 1/200 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-ABCE1 antibody [EPR15373(B)] - C-terminal (ab185548)
    Immunocytochemistry/ Immunofluorescence - Anti-ABCE1 antibody [EPR15373(B)] - C-terminal (ab185548)

    Immunofluorescent analysis of formaldehyde fixed 293 cells labeling ABCE1 using ab185548 at a 1/100 dilution. A Goat anti rabbit IgG (Alexa Fluor®488) was used as the secondary at a 1/200 dilution.

  • Flow Cytometry - Anti-ABCE1 antibody [EPR15373(B)] - C-terminal (ab185548)
    Flow Cytometry - Anti-ABCE1 antibody [EPR15373(B)] - C-terminal (ab185548)

    Flow Cytometry analysis of K562 cells labeling ABCE1 using ab185548 at a 1/220 dilution (pink). Goat anti rabbit IgG (FITC) used as the secondary at a 1/150 dilution. Isotype control Rabbit monoclonal IgG (green). Cells were fixed in 2% paraformaldehyde.

  • Immunoprecipitation - Anti-ABCE1 antibody [EPR15373(B)] - C-terminal (ab185548)
    Immunoprecipitation - Anti-ABCE1 antibody [EPR15373(B)] - C-terminal (ab185548)

    Western blot analysis of ABCE1 in immunoprecipitation pellets from 293 lysate (Lane 1) or 1XPBS (negative control) (Lane 2) using ab185548 at 1/50 dilution. Secondary antibody, Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at a 1/1500 dilution.

  • Anti-ABCE1 antibody [EPR15373(B)] - C-terminal (ab185548)
    Anti-ABCE1 antibody [EPR15373(B)] - C-terminal (ab185548)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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