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Signal Transduction Cytoskeleton / ECM Extracellular Matrix ECM Proteins Other ECM Proteins

Anti-DPT/TRAMP antibody [EPR22845-83] (ab255823)

Price and availability

321 638 ₸

Availability

Order now and get it on Friday March 05, 2021

Anti-DPT/TRAMP antibody [EPR22845-83] (ab255823)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22845-83] to DPT/TRAMP
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-DPT/TRAMP antibody [EPR22845-83]
    See all DPT/TRAMP primary antibodies
  • Description

    Rabbit monoclonal [EPR22845-83] to DPT/TRAMP
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human skin, Mouse skin, Rat skin, Human testis, Human liver and Human pancreas lysates.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR22845-83
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Extracellular Matrix
    • ECM Proteins
    • Other ECM Proteins

Images

  • Western blot - Anti-DPT/TRAMP antibody [EPR22845-83] (ab255823)
    Western blot - Anti-DPT/TRAMP antibody [EPR22845-83] (ab255823)
    All lanes : Anti-DPT/TRAMP antibody [EPR22845-83] (ab255823) at 1/1000 dilution

    Lane 1 : Human testis lysate
    Lane 2 : Human liver lysate
    Lane 3 : Human pancreas lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

    Observed band size: 22 kDa
    why is the actual band size different from the predicted?



    Blocking and Dilution Buffer and concentration: 5% NFDM/TBST.

    This blot was developed using a higher sensitivity ECL substrate.  There is weak expression in human testis, liver and pancreas.  The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID:10233760, 8082810).

    Exposure time: 125 seconds

  • Western blot - Anti-DPT/TRAMP antibody [EPR22845-83] (ab255823)
    Western blot - Anti-DPT/TRAMP antibody [EPR22845-83] (ab255823)
    All lanes : Anti-DPT/TRAMP antibody [EPR22845-83] (ab255823) at 1/1000 dilution

    Lane 1 : Human skin lysate
    Lane 2 : HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate
    Lane 3 : Mouse skin lysate
    Lane 4 : Rat skin lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/100000 dilution

    Observed band size: 22 kDa why is the actual band size different from the predicted?

  • Anti-DPT/TRAMP antibody [EPR22845-83] (ab255823)
    Anti-DPT/TRAMP antibody [EPR22845-83] (ab255823)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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