Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-DPT/TRAMP antibody [EPR22845-83] - BSA and Azide free (ab256824)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22845-83] to DPT/TRAMP - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-DPT/TRAMP antibody [EPR22845-83] - BSA and Azide free
    See all DPT/TRAMP primary antibodies

  • Description

    Rabbit monoclonal [EPR22845-83] to DPT/TRAMP - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human skin, Mouse skin, Rat skin, Human testis, Human liver and Human pancreas lysates.

  • General notes

    Ab256824 is the carrier-free version of ab255823. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab256824 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-DPT/TRAMP antibody [EPR22845-83] - BSA and Azide free (ab256824)
    Western blot - Anti-DPT/TRAMP antibody [EPR22845-83] - BSA and Azide free (ab256824)
    All lanes : Anti-DPT/TRAMP antibody [EPR22845-83] (ab255823) at 1/1000 dilution

    Lane 1 : Human testis lysate
    Lane 2 : Human liver lysate
    Lane 3 : Human pancreas lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

    Observed band size: 22 kDa
    why is the actual band size different from the predicted?



    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID:10233760 and 8082810).

    Negative control: HepG2 (PMID: 19916908).

    Exposure time:

    Lanes 1-3:15 seconds; Lanes 4:59 seconds

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255823).

  • Western blot - Anti-DPT/TRAMP antibody [EPR22845-83] - BSA and Azide free (ab256824)
    Western blot - Anti-DPT/TRAMP antibody [EPR22845-83] - BSA and Azide free (ab256824)
    All lanes : Anti-DPT/TRAMP antibody [EPR22845-83] (ab255823) at 1/1000 dilution

    Lane 1 : Human testis lysate
    Lane 2 : Human liver lysate
    Lane 3 : Human pancreas lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution


    Blocking and Dilution Buffer and concentration: 5% NFDM/TBST.

    This blot was developed using a higher sensitivity ECL substrate.  There is weak expression in human testis, liver and pancreas.  The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID:10233760, 8082810).

    Exposure time: 125 seconds

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255823).

  • Anti-DPT/TRAMP antibody [EPR22845-83] - BSA and Azide free (ab256824)
    Anti-DPT/TRAMP antibody [EPR22845-83] - BSA and Azide free (ab256824)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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