Anti-SMN/Gemin 1 antibody [EPR4429] - BSA and Azide free (ab247685)
![Anti-SMN/Gemin 1 antibody [EPR4429] - BSA and Azide free (ab247685)](https://www.abcam.com/ps/products/247/ab247685/Images/ab247685-445624-anti-smn-gemin-1-antibody-epr4429-bsa-and-azide-free-western-blot.jpg "Anti-SMN/Gemin 1 antibody [EPR4429] - BSA and Azide free (ab247685)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4429] to SMN/Gemin 1 - BSA and Azide free
- Suitable for: ICC, WB
- Reacts with: Human
Overview
-
Product name
Anti-SMN/Gemin 1 antibody [EPR4429] - BSA and Azide free
See all SMN/Gemin 1 primary antibodies -
Description
Rabbit monoclonal [EPR4429] to SMN/Gemin 1 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
-
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
General notes
ab247685 is the carrier-free version of ab108531 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab247685 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4429 -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-SMN/Gemin 1 antibody [EPR4429] - BSA and Azide free (ab247685)Anti-SMN/Gemin 1 antibody [EPR4429] (ab108531) at 1/1000 dilution (purified) + HeLa cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 32 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?This data was developed using ab108531, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
-
![Western blot - Anti-SMN/Gemin 1 antibody [EPR4429] - BSA and Azide free (ab247685)](https://www.abcam.com/ps/products/247/ab247685/Images/ab247685-410679-anti-smn-gemin-1-antibody-epr4429-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-SMN/Gemin 1 antibody [EPR4429] - BSA and Azide free (ab247685)All lanes : Anti-SMN/Gemin 1 antibody [EPR4429] (ab108531) at 1/1000 dilution (purified)
Lane 1 : K562 cell lysate
Lane 2 : HEK-293 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 32 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?This data was developed using ab108531, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
-
![Western blot - Anti-SMN/Gemin 1 antibody [EPR4429] - BSA and Azide free (ab247685)](https://www.abcam.com/ps/products/247/ab247685/Images/ab247685-408038-anti-smn-gemin-1-antibody-epr4429-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-SMN/Gemin 1 antibody [EPR4429] - BSA and Azide free (ab247685)Anti-SMN/Gemin 1 antibody [EPR4429] (ab108531) at 1/5000 dilution (purified) + HepG2 cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 32 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?This data was developed using ab108531, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
-
![Western blot - Anti-SMN/Gemin 1 antibody [EPR4429] - BSA and Azide free (ab247685)](https://www.abcam.com/ps/products/247/ab247685/Images/ab247685-405924-anti-smn-gemin-1-antibody-epr4429-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-SMN/Gemin 1 antibody [EPR4429] - BSA and Azide free (ab247685)Lane 1 : Anti-SMN/Gemin 1 antibody [EPR4429] (ab108531) at 1/1000 dilution (unpurified)
Lanes 2-4 : Anti-SMN/Gemin 1 antibody [EPR4429] (ab108531) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : K562 cell lysate
Lane 4 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 32 kDaThis data was developed using ab108531, the same antibody clone in a different buffer formulation.
-
![Immunocytochemistry - Anti-SMN/Gemin 1 antibody [EPR4429] - BSA and Azide free (ab247685)](https://www.abcam.com/ps/products/247/ab247685/Images/ab247685-1-anti-smn-gemin-1-antibody-epr4429-bsa-and-azide-free-immunocytochemistry.jpg)
Immunocytochemistry - Anti-SMN/Gemin 1 antibody [EPR4429] - BSA and Azide free (ab247685)This data was developed using ab108531, the same antibody clone in a different buffer formulation.Immunocytochemistry/Immunofluorescence analysis of SH-SY5Y cells labelling Gemin 1 with purified ab108531 at 1/250. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/500) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500) were also used.Control 1: primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500). -
![Anti-SMN/Gemin 1 antibody [EPR4429] - BSA and Azide free (ab247685)](https://www.abcam.com/ps/products/247/ab247685/Images/ab247685-5-benefits-of-recombinant-antibodies.png)
Anti-SMN/Gemin 1 antibody [EPR4429] - BSA and Azide free (ab247685)