Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SMN/Gemin 1 antibody [EPR4429] - BSA and Azide free (ab247685)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4429] to SMN/Gemin 1 - BSA and Azide free
  • Suitable for: ICC, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-SMN/Gemin 1 antibody [EPR4429] - BSA and Azide free
    See all SMN/Gemin 1 primary antibodies

  • Description

    Rabbit monoclonal [EPR4429] to SMN/Gemin 1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab247685 is the carrier-free version of ab108531 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab247685 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-SMN/Gemin 1 antibody [EPR4429] - BSA and Azide free (ab247685)
    Western blot - Anti-SMN/Gemin 1 antibody [EPR4429] - BSA and Azide free (ab247685)
    Anti-SMN/Gemin 1 antibody [EPR4429] (ab108531) at 1/1000 dilution (purified) + HeLa cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 32 kDa
    Observed band size: 35 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab108531, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-SMN/Gemin 1 antibody [EPR4429] - BSA and Azide free (ab247685)
    Western blot - Anti-SMN/Gemin 1 antibody [EPR4429] - BSA and Azide free (ab247685)
    All lanes : Anti-SMN/Gemin 1 antibody [EPR4429] (ab108531) at 1/1000 dilution (purified)

    Lane 1 : K562 cell lysate
    Lane 2 : HEK-293 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 32 kDa
    Observed band size: 35 kDa why is the actual band size different from the predicted?



    This data was developed using ab108531, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-SMN/Gemin 1 antibody [EPR4429] - BSA and Azide free (ab247685)
    Western blot - Anti-SMN/Gemin 1 antibody [EPR4429] - BSA and Azide free (ab247685)
    Anti-SMN/Gemin 1 antibody [EPR4429] (ab108531) at 1/5000 dilution (purified) + HepG2 cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 32 kDa
    Observed band size: 35 kDa why is the actual band size different from the predicted?



    This data was developed using ab108531, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-SMN/Gemin 1 antibody [EPR4429] - BSA and Azide free (ab247685)
    Western blot - Anti-SMN/Gemin 1 antibody [EPR4429] - BSA and Azide free (ab247685)
    Lane 1 : Anti-SMN/Gemin 1 antibody [EPR4429] (ab108531) at 1/1000 dilution (unpurified)
    Lanes 2-4 : Anti-SMN/Gemin 1 antibody [EPR4429] (ab108531) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : K562 cell lysate
    Lane 4 : 293T cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

    Predicted band size: 32 kDa



    This data was developed using ab108531, the same antibody clone in a different buffer formulation.

  • Immunocytochemistry - Anti-SMN/Gemin 1 antibody [EPR4429] - BSA and Azide free (ab247685)
    Immunocytochemistry - Anti-SMN/Gemin 1 antibody [EPR4429] - BSA and Azide free (ab247685)
    This data was developed using ab108531, the same antibody clone in a different buffer formulation.Immunocytochemistry/Immunofluorescence analysis of SH-SY5Y cells labelling Gemin 1 with purified ab108531 at 1/250. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/500) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500) were also used.Control 1: primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500).
  • Anti-SMN/Gemin 1 antibody [EPR4429] - BSA and Azide free (ab247685)
    Anti-SMN/Gemin 1 antibody [EPR4429] - BSA and Azide free (ab247685)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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