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Neuroscience Neurology process Neural Signal Transduction

Anti-NDRG1 (phospho S330) antibody [EPR5594] (ab124713)

Price and availability

251 280 ₸

Availability

Order now and get it on Thursday March 04, 2021

Anti-NDRG1 (phospho S330) antibody [EPR5594] (ab124713)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5594] to NDRG1 (phospho S330)
  • Suitable for: WB, IHC-P
  • Reacts with: Human

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Overview

  • Product name

    Anti-NDRG1 (phospho S330) antibody [EPR5594]
    See all NDRG1 primary antibodies
  • Description

    Rabbit monoclonal [EPR5594] to NDRG1 (phospho S330)
  • Host species

    Rabbit
  • Specificity

    This antibody only detects NDRG1 when phosphorylated at Serine 330.
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    A phospho specific peptide corresponding to residues surrounding Serine 330 of Human NDRG1

  • Positive control

    • Calyculin A treated 293T cell lysates; Human kidney tissue
  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Dissociation constant (KD)

    KD = 5.30 x 10 -11 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR5594
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurology process
    • Neural Signal Transduction
    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Other
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Cancer susceptibility
    • Proto-oncogenes
    • Cancer
    • Oncoproteins/suppressors
    • Oncoproteins
    • Signal transducers
    • Cancer
    • Invasion/microenvironment
    • Angiogenesis
    • Other

Images

  • Western blot - Anti-NDRG1 (phospho S330) antibody [EPR5594] (ab124713)
    Western blot - Anti-NDRG1 (phospho S330) antibody [EPR5594] (ab124713)
    All lanes : Anti-NDRG1 (phospho S330) antibody [EPR5594] (ab124713) at 1/10000 dilution

    Lane 1 : Untreated 293T cell lysates
    Lane 2 : Calyculin A treated 293T cell lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 43 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDRG1 (phospho S330) antibody [EPR5594] (ab124713)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDRG1 (phospho S330) antibody [EPR5594] (ab124713)

    ab124713, at 1/100, staining NDRG1 in paraffin embedded Human kidney tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-NDRG1 (phospho S330) antibody [EPR5594] (ab124713)
    OI-RD Scanning - Anti-NDRG1 (phospho S330) antibody [EPR5594] (ab124713)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-NDRG1 (phospho S330) antibody [EPR5594] (ab124713)
    Anti-NDRG1 (phospho S330) antibody [EPR5594] (ab124713)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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