Anti-NRG1 type III antibody (ab23248)
Key features and details
- Rabbit polyclonal to NRG1 type III
- Suitable for: IHC-Fr, WB
- Reacts with: Mouse, Rat
- Isotype: IgG
Overview
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Product name
Anti-NRG1 type III antibody -
Description
Rabbit polyclonal to NRG1 type III -
Host species
Rabbit -
Specificity
ab23248 detects ~50-55kDa band in rat sciatic nerve, rat and mouse brain lysate. IHC detection with ab23248 in mouse DRG shows a primarily nuclear localisation of the protein rather than the expected cytoplasmic staining (See Bao and Wang references). -
Tested applications
Suitable for: IHC-Fr, WBmore details -
Species reactivity
Reacts with: Mouse, Rat
Predicted to work with: Chicken, Human
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Immunogen
Synthetic peptide corresponding to Human NRG1 type III aa 50-150 (internal sequence) conjugated to keyhole limpet haemocyanin.
(Peptide available asab23378)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab23248 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes IHC-Fr Use a concentration of 0.34 µg/ml. WB Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa. Target
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Relevance
NRG1 type III is a sensory and motor neuron-derived factor isoform of Neuregulin 1, specifically expressed in the nervous system: spinal cord motor neurons, dorsal root ganglion neurons, and brain. NRG1 type III is the predominant isoform expressed in sensory and motor neurons. The Nrg1 gene encodes more than 15 transmembrane and secreted protein isoforms, generated by alternative promoter usage and mRNA splicing. Nrg1 subtypes I through III share the epidermal growth factor-like signaling domain and are defined by different amino-termini. The N-terminal cysteine-rich domain (CRD), as found in the sensory and motor neuronderived factor (SMDF), defines Nrg1 type III, which in embryonic development is responsible for survival of Schwann cell precursors. -
Cellular localization
Cell Membrane and Secreted -
Database links
- Entrez Gene: 3084 Human
- Entrez Gene: 211323 Mouse
- Entrez Gene: 112400 Rat
- SwissProt: Q02297 Human
- SwissProt: Q15491 Human
- SwissProt: P43322 Rat
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Alternative names
- Heregulin antibody
- HRG antibody
- HRG1 antibody
see all
Images
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Immunohistochemistry (Frozen sections) - Anti-NRG1 type III antibody (ab23248)This image is courtesy of David Bennett, Kings College London, United Kingdom
ab23248 detecting NRG1 Type III in mouse L4/5 dorsal root ganglion (DRG) tissue (perfused with 4% paraformaldehyde and cryoprotected overnight, cut on a cryostat at 15µm). Tissue was incubated in blocking serum (normal goat serum) for 1hr, washed 3XPBS before primary antibody incubation in PBS with triton (0.1%) overnight (ab23248 was used at 1/800 = 0.34µg/ml). Secondary antibody used was goat anti-rabbit Alexa 546 (1/1000). The image shown is taken with a X20 objective and shows mostly nuclear NRG1 Type III staining with some cytomplasmic staining (nuclear counterstain with DAPI confirmed this - not shown).
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Western blot - Anti-NRG1 type III antibody (ab23248)All lanes : Anti-NRG1 type III antibody (ab23248) at 1 µg/ml
Lane 1 : Mouse Sciatic Nerve Lysate
Lane 2 : Mouse Brain
Lane 3 : Brain (Rat) Tissue Lysate (ab7942)
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Rabbit IgG secondary antibody (ab28446) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 27,52,74,75 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted?ab23248 picks up an ~50kDa band in WB when tested on rat sciatic nerve, rat brain or mouse brain. This band size concurrs with an entry for NRG1 in both Expasy and NCBI databases (Q3URY7). Carroll et al., (J Neurosci. 1997 Mar 1;17(5):1642-59) also detect a 50kDa band in dorsal root ganglion and spinal cord. However, it is surprising that we do not detect more bands; it is likely that ab23248 does not pick up all NRG1 type III isoforms or that there is very restricted expression in the tissues tested here.
Protocols
Datasheets and documents
References (4)
ab23248 has been referenced in 4 publications.
- Chen S et al. Neuregulin-1 Accelerates Functional Motor Recovery by Improving Motoneuron Survival After Brachial Plexus Root Avulsion in Mice. Neuroscience 404:510-518 (2019). PubMed: 30731156
- Nguyen TV et al. Alzheimer's associated amyloid and tau deposition co-localizes with a homeostatic myelin repair pathway in two mouse models of post-stroke mixed dementia. Acta Neuropathol Commun 6:100 (2018). PubMed: 30249297
- Yue Y et al. Low-intensity pulsed ultrasound upregulates pro-myelination indicators of Schwann cells enhanced by co-culture with adipose-derived stem cells. Cell Prolif 49:720-728 (2016). PubMed: 27625295
- Sango K et al. Myelination in coculture of established neuronal and Schwann cell lines. Histochem Cell Biol 137:829-39 (2012). ICC/IF ; Rat . PubMed: 22366958
Images
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Immunohistochemistry (Frozen sections) - Anti-NRG1 type III antibody (ab23248) This image is courtesy of David Bennett, Kings College London, United Kingdom
ab23248 detecting NRG1 Type III in mouse L4/5 dorsal root ganglion (DRG) tissue (perfused with 4% paraformaldehyde and cryoprotected overnight, cut on a cryostat at 15µm). Tissue was incubated in blocking serum (normal goat serum) for 1hr, washed 3XPBS before primary antibody incubation in PBS with triton (0.1%) overnight (ab23248 was used at 1/800 = 0.34µg/ml). Secondary antibody used was goat anti-rabbit Alexa 546 (1/1000). The image shown is taken with a X20 objective and shows mostly nuclear NRG1 Type III staining with some cytomplasmic staining (nuclear counterstain with DAPI confirmed this - not shown).
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Western blot - Anti-NRG1 type III antibody (ab23248)All lanes : Anti-NRG1 type III antibody (ab23248) at 1 µg/ml
Lane 1 : Mouse Sciatic Nerve Lysate
Lane 2 : Mouse Brain
Lane 3 : Brain (Rat) Tissue Lysate (ab7942)
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Rabbit IgG secondary antibody (ab28446) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 27,52,74,75 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted?ab23248 picks up an ~50kDa band in WB when tested on rat sciatic nerve, rat brain or mouse brain. This band size concurrs with an entry for NRG1 in both Expasy and NCBI databases (Q3URY7). Carroll et al., (J Neurosci. 1997 Mar 1;17(5):1642-59) also detect a 50kDa band in dorsal root ganglion and spinal cord. However, it is surprising that we do not detect more bands; it is likely that ab23248 does not pick up all NRG1 type III isoforms or that there is very restricted expression in the tissues tested here.
