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Signal Transduction Signaling Pathway Nuclear Signaling STATs

Anti-STAT5a antibody (ab223682)

Anti-STAT5a antibody (ab223682)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Goat polyclonal to STAT5a
  • Suitable for: IHC-P, WB
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-STAT5a antibody
    See all STAT5a primary antibodies
  • Description

    Goat polyclonal to STAT5a
  • Host species

    Goat
  • Specificity

    ab223682 is not expected to cross-react with STAT5B.
  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Human STAT5a aa 650-750 (internal sequence) (Cysteine residue). NP_003143.2.
    Database link: P42229

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • WB: K562 cell lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 0.5% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab223682 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • STATs
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors

Images

  • Western blot - Anti-STAT5a antibody (ab223682)
    Western blot - Anti-STAT5a antibody (ab223682)
    Anti-STAT5a antibody (ab223682) at 0.1 µg/ml + K562 (human chronic myelogenous leukemia cell line from bone marrow) cell lysate in RIPA buffer at 35 µg

    Developed using the ECL technique.

    Predicted band size: 90 kDa
    Observed band size: 100 kDa
    why is the actual band size different from the predicted?



    Primary incubation was 1 hour.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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