Anti-RSU1 antibody [EPR16127] (ab191409)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16127] to RSU1
- Suitable for: IP, WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-RSU1 antibody [EPR16127]
See all RSU1 primary antibodies -
Description
Rabbit monoclonal [EPR16127] to RSU1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB MouseHuman -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Caco 2, Jurkat, HepG2, C6, Raw264.7, PC12 and NIH 3T3 cell lysates; Human placenta tissue lysate, Mouse brain, heart, kidney and spleen tissue lysates; Rat brain, heart, kidney and spleen tissue lysates
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR16127 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-RSU1 antibody [EPR16127] (ab191409) at 1/1000 dilution
Lane 1 : Human placenta tissue lysate
Lane 2 : Caco 2 cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 32 kDa
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Anti-RSU1 antibody [EPR16127] (ab191409) at 1/10000 dilution + Caco 2 cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 32 kDa
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All lanes : Anti-RSU1 antibody [EPR16127] (ab191409) at 1/1000 dilution
Lane 1 : Mouse brain tissue lysate
Lane 2 : Mouse heart tissue lysate
Lane 3 : Mouse kidney tissue lysate
Lane 4 : Mouse spleen tissue lysate
Lane 5 : Rat brain tissue lysate
Lane 6 : Rat heart tissue lysate
Lane 7 : Rat kidney tissue lysate
Lane 8 : Rat spleen tissue lysate
Lane 9 : C6 cell lysate
Lane 10 : Raw264.7 cell lysate
Lane 11 : PC12 cell lysate
Lane 12 : NIH 3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 32 kDa
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Immunoprecipitation analysis of Caco 2 cell lysate labeling RSU1 using ab191409 at 1/100 dilution (Lane 1). An anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 was used as secondary antibody. Lane 2: PBS instead of Caco 2 cell lysate.
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