Antibodies, Proteins, Kits and Reagents for Life Science

284 784 ₸ Product size

100 µl 284 784 ₸

Order now and get it on Thursday March 04, 2021

Anti-RSU1 antibody [EPR16127] (ab191409)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR16127] to RSU1
Suitable for: IP, WB
Reacts with: Mouse, Rat, Human

Product name

Anti-RSU1 antibody [EPR16127]
See all RSU1 primary antibodies
Description

Rabbit monoclonal [EPR16127] to RSU1
Host species

Rabbit
Tested Applications & Species

Application Species
IP
Human

WB
Mouse

Human

See all applications and species data
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- Caco 2, Jurkat, HepG2, C6, Raw264.7, PC12 and NIH 3T3 cell lysates; Human placenta tissue lysate, Mouse brain, heart, kidney and spleen tissue lysates; Rat brain, heart, kidney and spleen tissue lysates
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IP	Human
WB	Mouse Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR16127
Isotype

IgG
Research areas

Western blot - Anti-RSU1 antibody [EPR16127] (ab191409)

All lanes : Anti-RSU1 antibody [EPR16127] (ab191409) at 1/1000 dilution

Lane 1 : Human placenta tissue lysate
Lane 2 : Caco 2 cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : HepG2 cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 32 kDa
Western blot - Anti-RSU1 antibody [EPR16127] (ab191409)

Anti-RSU1 antibody [EPR16127] (ab191409) at 1/10000 dilution + Caco 2 cell lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 32 kDa
Western blot - Anti-RSU1 antibody [EPR16127] (ab191409)

All lanes : Anti-RSU1 antibody [EPR16127] (ab191409) at 1/1000 dilution

Lane 1 : Mouse brain tissue lysate
Lane 2 : Mouse heart tissue lysate
Lane 3 : Mouse kidney tissue lysate
Lane 4 : Mouse spleen tissue lysate
Lane 5 : Rat brain tissue lysate
Lane 6 : Rat heart tissue lysate
Lane 7 : Rat kidney tissue lysate
Lane 8 : Rat spleen tissue lysate
Lane 9 : C6 cell lysate
Lane 10 : Raw264.7 cell lysate
Lane 11 : PC12 cell lysate
Lane 12 : NIH 3T3 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 32 kDa
Immunoprecipitation - Anti-RSU1 antibody [EPR16127] (ab191409)

Immunoprecipitation analysis of Caco 2 cell lysate labeling RSU1 using ab191409 at 1/100 dilution (Lane 1). An anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 was used as secondary antibody. Lane 2: PBS instead of Caco 2 cell lysate.
Anti-RSU1 antibody [EPR16127] (ab191409)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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