Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-NDRG1 antibody [EPR5593] - BSA and Azide free (ab226082)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5593] to NDRG1 - BSA and Azide free
  • Suitable for: WB, IP, IHC-P, ICC/IF
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-NDRG1 antibody [EPR5593] - BSA and Azide free
    See all NDRG1 primary antibodies

  • Description

    Rabbit monoclonal [EPR5593] to NDRG1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IP, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human colon tissue, cervical carcinoma tissue, colonic adenocarcinoma tissue, normal kidney tissue and breast carcinoma tissue. ICC/IF: HeLa. IP: HeLa.

  • General notes

    ab226082 is the carrier-free version of ab124689. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab226082 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDRG1 antibody [EPR5593] - BSA and Azide free (ab226082)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDRG1 antibody [EPR5593] - BSA and Azide free (ab226082)

    ab124689 at 1/100 dilution, staining NDRG1 in Formalin fixed Paraffin-embedded human colon tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124689).

  • Immunocytochemistry/ Immunofluorescence - Anti-NDRG1 antibody [EPR5593] - BSA and Azide free (ab226082)
    Immunocytochemistry/ Immunofluorescence - Anti-NDRG1 antibody [EPR5593] - BSA and Azide free (ab226082)

    ab124689, at 1/100 dilution, staining NDRG1 in HeLa cells by Immunofluorescence.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124689).

  • Immunoprecipitation - Anti-NDRG1 antibody [EPR5593] - BSA and Azide free (ab226082)
    Immunoprecipitation - Anti-NDRG1 antibody [EPR5593] - BSA and Azide free (ab226082)

    Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10µg.

    Lane 2 (+): ab124689 & HeLa whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab124689 in HeLa whole cell lysate

    ab124689 (Purified) at 1/50 dilution (20µg/ml) immunoprecipitating NDRG1 in HeLa whole cell lysate. For western blotting, ab124689 at 1/500 dilution (1.86 µg/mL) and VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/1000 dilution.

    Blocking and diluting buffer: 5% NFDM /TBST .

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124689).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDRG1 antibody [EPR5593] - BSA and Azide free (ab226082)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDRG1 antibody [EPR5593] - BSA and Azide free (ab226082)

    ab124689 showing positive staining in human cervical carcinoma tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124689).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDRG1 antibody [EPR5593] - BSA and Azide free (ab226082)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDRG1 antibody [EPR5593] - BSA and Azide free (ab226082)

    ab124689 showing positive staining in human colonic adenocarcinoma tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124689).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDRG1 antibody [EPR5593] - BSA and Azide free (ab226082)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDRG1 antibody [EPR5593] - BSA and Azide free (ab226082)

    ab124689 showing positive staining in human normal kidney tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124689).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDRG1 antibody [EPR5593] - BSA and Azide free (ab226082)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDRG1 antibody [EPR5593] - BSA and Azide free (ab226082)

    ab124689 showing positive staining in human breast carcinoma tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124689).

  • OI-RD Scanning - Anti-NDRG1 antibody [EPR5593] - BSA and Azide free (ab226082)
    OI-RD Scanning - Anti-NDRG1 antibody [EPR5593] - BSA and Azide free (ab226082)

    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124689).

  • Anti-NDRG1 antibody [EPR5593] - BSA and Azide free (ab226082)
    Anti-NDRG1 antibody [EPR5593] - BSA and Azide free (ab226082)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-NDRG1 antibody [EPR5593] - BSA and Azide free (ab226082)

© 2021 Astana Biomed Group LLP. All rights reserved.