Anti-SMN/Gemin 1 antibody (ab231351)
Key features and details
- Rabbit polyclonal to SMN/Gemin 1
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SMN/Gemin 1 antibody
See all SMN/Gemin 1 primary antibodies -
Description
Rabbit polyclonal to SMN/Gemin 1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Recombinant full length protein (His-tag) corresponding to Human SMN/Gemin 1 aa 1 to the C-terminus. Expressed in E.coli. N-terminal tag.
Database link: Q16637-1 -
Positive control
- ICC/IF: HeLa cells. WB: HeLa, HEK-293T and HepG2 cell lysate; Recombinant human SMN/Gemin 1 protein.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.05% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-SMN/Gemin 1 antibody (ab231351)
FITC staining of formalin-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling SMN/Gemin 1 (green)using ab231351 at 20 μg/ml in ICC/IF.
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Western blot - Anti-SMN/Gemin 1 antibody (ab231351)All lanes : Anti-SMN/Gemin 1 antibody (ab231351) at 3 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Secondary
All lanes : HRP-Linked Guinea pig Anti-Rabbit Ab at 1/2000 dilution
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Western blot - Anti-SMN/Gemin 1 antibody (ab231351)Anti-SMN/Gemin 1 antibody (ab231351) at 3 µg/ml + Recombinant human SMN/Gemin 1 protein
