Anti-SMN/Gemin 1 antibody (ab232784)
Key features and details
- Rabbit polyclonal to SMN/Gemin 1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-SMN/Gemin 1 antibody
See all SMN/Gemin 1 primary antibodies -
Description
Rabbit polyclonal to SMN/Gemin 1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Cynomolgus monkey, Orangutan
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Immunogen
Recombinant fragment (His-tag) corresponding to Human SMN/Gemin 1 aa 1 to the C-terminus. (Expressed in E.coli).
Database link: Q16637 -
Positive control
- IHC-P: Human liver, kidney, esophagus and stomach tissues. ICC/IF: HeLa cells. WB: Recombinant human SMN/Gemin 1 protein; Human serum; HeLa, HepG2, HEK-293T cell lysates; Mouse testis lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab232784 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-SMN/Gemin 1 antibody (ab232784)Anti-SMN/Gemin 1 antibody (ab232784) at 2 µg/ml + Recombinant human SMN/Gemin 1 protein.
Predicted band size: 32 kDa
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Western blot - Anti-SMN/Gemin 1 antibody (ab232784)All lanes : Anti-SMN/Gemin 1 antibody (ab232784) at 2 µg/ml
Lane 1 : Human serum
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 3 : HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 5 : Mouse testis lysate
Predicted band size: 32 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMN/Gemin 1 antibody (ab232784)Paraffin-embedded human liver tissue stained for SMN/Gemin 1 using ab232784 at 20 µg/ml in immunohistochemical analysis. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMN/Gemin 1 antibody (ab232784)Paraffin-embedded human kidney tissue stained for SMN/Gemin 1 using ab232784 at 20 µg/ml in immunohistochemical analysis. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMN/Gemin 1 antibody (ab232784)Paraffin-embedded human esophagus tissue stained for SMN/Gemin 1 using ab232784 at 20 µg/ml in immunohistochemical analysis. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMN/Gemin 1 antibody (ab232784)Paraffin-embedded human stomach tissue stained for SMN/Gemin 1 using ab232784 at 20 µg/ml in immunohistochemical analysis. DAB staining.
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Immunocytochemistry/ Immunofluorescence - Anti-SMN/Gemin 1 antibody (ab232784)HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for SMN/Gemin 1 (green) using ab232784 at 20 µg/ml in ICC/IF, followed by FITC staining.
