All lanes : Anti-Nck 1/2 antibody [AF6D7] (ab80620) at 1/2000 dilution

Lane 1 : HeLa Cell Lysate
Lane 2 : 239T Cell Lysate
Lane 3 : PC12 Cell Lysate
Lane 4 : NIH3T3 Cell Lysate

Predicted band size: 47 kDa

Ladder M.W(kDa): 7, 17, 25, 30, 46, 58, 80, 175

Ladder M.W(kDa): 7, 17, 25, 30, 46, 58, 80, 175

All lanes :

Lane 1 : A431 Cell Lysate 200ug
Lane 2 : Precipitated from A431 Cell Lysate 200ug using ab80620 at 2ug
Lane 3 : Precipitated from A431 Cell Lysate 200ug using ab80620 at 5ug
Lane 4 : Precipitated from PBS using ab80620 at 5ug

All lanes : Anti-Nck 1/2 antibody [AF6D7] (ab80620) at 1/5000 dilution

Lane 1 : NCK1 Recombinant Protein
Lane 2 : NCK2 Recombinant Protein

Predicted band size: 47 kDa

Ladder M.W(kDa): 7, 17, 25, 30, 46, 58, 80, 175

All lanes : Anti-Nck 1/2 antibody [AF6D7] (ab80620) at 1/1000 dilution

Lane 1 : HeLa cell lysate
Lane 2 : U937 cell lysate
Lane 3 : A431 cell lysate
Lane 4 : SK-N-MC cell lysate
Lane 5 : Ramos cell lysate
Lane 6 : PC12 cell lysate

Predicted band size: 47 kDa