Human SFN (14-3-3 sigma) knockout HeLa cell lysate (ab257288)
Properties
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Storage instructions
Store at -80°C. Please refer to protocols. -
Components 1 kit ab260878 - Human SFN knockout HeLa cell lysate 1 x 100µg ab255929 - Human wild-type HeLa cell lysate 1 x 100µg -
Research areas
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Cell type
epithelial -
Disease
Adenocarcinoma -
Gender
Female -
STR Analysis
Amelogenin X D5S818: 11, 12 D13S317: 12, 13.3 D7S820: 8, 12 D16S539: 9, 10 vWA: 16, 18 TH01: 7 TPOX: 8, 12 CSF1PO: 9, 10
Images
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Western blot - Human SFN knockout HeLa cell lysate (ab257288)
Lane 1: Wild-type HeLa cell lysate (20 µg)
Lane 2: SFN knockout HeLa cell lysate (20 µg)
Lane 3: A431 cell lysate (20 µg)
Lanes 1-3: Merged signal (red and green). Green - ab14123 observed at 28 kDa. Red - loading control, ab52866 observed at 50 kDa.
ab14123 Anti-14-3-3 sigma/SFN antibody [1433S01] was shown to specifically react with 14-3-3 sigma/SFN in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab261779 (knockout cell lysate ab257288) was used. Wild-type and 14-3-3 sigma/SFN knockout samples were subjected to SDS-PAGE. ab14123 and Anti-alpha Tubulin antibody [EP1332Y] - Loading Control (ab52866) were incubated overnight at 4°C at 1 in 200 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
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Sanger Sequencing - Human SFN knockout HeLa cell lysate (ab257288)Allele-1: 2 bp deletion in exon 1
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Sanger Sequencing - Human SFN knockout HeLa cell lysate (ab257288)Allele-2: 1 bp deletion in exon 1