Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ROR alpha/RORA antibody [EPR23719-48] - BSA and Azide free (ab278109)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23719-48] to ROR alpha/RORA - BSA and Azide free
  • Suitable for: IP, IHC-P, IHC-Fr
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-ROR alpha/RORA antibody [EPR23719-48] - BSA and Azide free
    See all ROR alpha/RORA primary antibodies

  • Description

    Rabbit monoclonal [EPR23719-48] to ROR alpha/RORA - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, IHC-P, IHC-Frmore details
    Unsuitable for: WB

  • Species reactivity

    Reacts with: Mouse, Rat

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-Fr: Mouse Cerebellum tissue IHC-P: Mouse Cerebellum tissue; Rat Cerebellum tissue. IP: Mouse cerebellum tissue lysate; Rat cerebellum tissue lysate.

  • General notes

    ab278109 is the carrier-free version of ab278099. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab278109 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROR alpha/RORA antibody [EPR23719-48] (ab278109)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROR alpha/RORA antibody [EPR23719-48] (ab278109)

    This data was developed using ab278099, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse cerebellum tissue labeling ROR alpha/RORA with ab278099 at 1/800 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on mouse cerebellum ( PMID:14966198). The section was incubated with ab278099 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunoprecipitation - Anti-ROR alpha/RORA antibody [EPR23719-48] (ab278109)
    Immunoprecipitation - Anti-ROR alpha/RORA antibody [EPR23719-48] (ab278109)

    This data was developed using ab278099, the same antibody clone in a different buffer formulation.

    ROR alpha/RORA was immunoprecipitated from 0.35 mg Mouse cerebellum tissue lysate with ab278099 at 1/30 dilution. Western blot was performed on the immunoprecipitate using ab278099 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: Mouse cerebellum tissue lysate 10ug

    Lane 2: ab278099 IP in Mouse cerebellum tissue lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab278099 in mouse cerebellum tissue lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes

  • Immunohistochemistry (Frozen sections) - Anti-ROR alpha/RORA antibody [EPR23719-48] (ab278109)
    Immunohistochemistry (Frozen sections) - Anti-ROR alpha/RORA antibody [EPR23719-48] (ab278109)

    This data was developed using ab278099, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cerebellum tissue labeling ROR alpha/RORA with ab278099 at 1/50 dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on mouse cerebellum is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Immunoprecipitation - Anti-ROR alpha/RORA antibody [EPR23719-48] (ab278109)
    Immunoprecipitation - Anti-ROR alpha/RORA antibody [EPR23719-48] (ab278109)

    This data was developed using ab278099, the same antibody clone in a different buffer formulation.

    ROR alpha/RORA was immunoprecipitated from 0.35 mg Rat cerebellum tissue lysate with ab278099 at 1/30 dilution. Western blot was performed on the immunoprecipitate using ab278099 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: Rat cerebellum tissue lysate 10ug

    Lane 2: ab278099 IP in Rat cerebellum tissue lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab278099 in rat cerebellum tissue lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROR alpha/RORA antibody [EPR23719-48] (ab278109)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROR alpha/RORA antibody [EPR23719-48] (ab278109)

    This data was developed using ab278099, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Rat cerebellum tissue labeling ROR alpha/RORA with ab278099 at 1/800 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on rat cerebellum. The section was incubated with ab278099 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry (Frozen sections) - Anti-ROR alpha/RORA antibody [EPR23719-48] (ab278109)
    Immunohistochemistry (Frozen sections) - Anti-ROR alpha/RORA antibody [EPR23719-48] (ab278109)

    This data was developed using ab278099, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse kidney tissue labeling ROR alpha/RORA with ab278099 at 1/50 dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Negative control: no staining on mouse kidney (PMID: 9226375) is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

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