Anti-YWHAE antibody (ab182656)
Key features and details
- Rabbit polyclonal to YWHAE
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-YWHAE antibody
See all YWHAE primary antibodies -
Description
Rabbit polyclonal to YWHAE -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Sheep, Chicken, Cow -
Immunogen
Recombinant full length protein corresponding to Human YWHAE aa 1 to the C-terminus. (With tag; BC000179).
Database link: P62258 -
Positive control
- Mouse brain tissue lysate; HT29, 293T and HeLa cell lysates; Human colon cancer tissue.
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General notes
This product was previously labelled as 14-3-3 epsilon
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine)
Note: PBS without Mg2+ and Ca2+. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue lableing YWHAE with ab182656 at 1/10 dilution (left panel). Right panel is treated with the immunogen.
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All lanes : Anti-YWHAE antibody (ab182656) at 1/250 dilution
Lane 1 : HT29 cell lysate
Lane 2 : Mouse brain tissue lysate
Lane 3 : 293T cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Donkey
anti Rabbit IgG - H&L (HRP) at 1/3000 dilution
Developed using the ECL technique.
Predicted band size: 29 kDa
Exposure time: 1 second10% SDS PAGE