Anti-YWHAE antibody (ab137862)
Key features and details
- Rabbit polyclonal to YWHAE
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-YWHAE antibody
See all YWHAE primary antibodies -
Description
Rabbit polyclonal to YWHAE -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Chicken, Cow, Xenopus laevis, Xenopus tropicalis -
Immunogen
Recombinant fragment corresponding to Human YWHAE aa 58-255.
Database link: NP_006752 -
Positive control
- 293T, H1299, HeLa, HepG2, Molt4, Raji, Mouse brain whole cell lysates, MDA MB231 xenograft tissue, A431 cells and whole cell lysate.
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General notes
This product was previously labelled as 14-3-3 epsilon
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Anti-YWHAE antibody (ab137862) at 1/1000 dilution + H1299 whole cell lysate at 30 µg
Predicted band size: 29 kDa
12% SDS PAGE -
Anti-YWHAE antibody (ab137862) at 1/10000 dilution + Mouse brain whole cell lysate at 20 µg
Predicted band size: 29 kDa
12% SDS PAGE -
Immunofluorescence analysis of methanol fixed A431 cells labelling YWHAE with 137862 at 1/200. Lower panel shows costaining with Hoechst 33342.
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Immunohistochemical analysis of paraffin embedded MDA MB231 xenograft tissue labelling YWHAE with ab137862 at 1/500.