Anti-PHOX2B antibody - N-terminal (ab227719)
Key features and details
- Rabbit polyclonal to PHOX2B - N-terminal
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PHOX2B antibody - N-terminal
See all PHOX2B primary antibodies -
Description
Rabbit polyclonal to PHOX2B - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Xenopus laevis -
Immunogen
Synthetic peptide within Human PHOX2B (N terminal). The exact sequence is proprietary. Conjugated to a protein carrier.
Database link: Q99453 -
Positive control
- IP: IMR32 whole cell extract. WB: IMR32, SK-N-AS and SH-SY5Y whole cell extracts.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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PHOX2B was immunoprecipitated from IMR32 (human neuroblast cell line) whole cell extract with 5 µg ab227719. Western blot was performed from the immunoprecipitate using ab227719. Anti-Rabbit IgG was used as a secondary reagent.
Lane 1: Control IgG IP in IMR32 whole cell extract.
Lane 2: ab227719 IP in IMR32 whole cell extract.
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All lanes : Anti-PHOX2B antibody - N-terminal (ab227719) at 1/1000 dilution
Lane 1 : IMR32 (human neuroblast cell line) whole cell extract
Lane 2 : SK-N-AS (human neuroblastoma cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 32 kDa12% SDS-PAGE gel.
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Anti-PHOX2B antibody - N-terminal (ab227719) at 1/1000 dilution + SH-SY5Y (human neuroblastoma cell line from bone marrow) whole cell extract at 30 µg
Predicted band size: 32 kDa12% SDS-PAGE gel.