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Neuroscience Neurology process Neural Signal Transduction

Anti-PHOX2B antibody - N-terminal (ab227719)

Anti-PHOX2B antibody - N-terminal (ab227719)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to PHOX2B - N-terminal
  • Suitable for: WB, IP
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-PHOX2B antibody - N-terminal
    See all PHOX2B primary antibodies
  • Description

    Rabbit polyclonal to PHOX2B - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Xenopus laevis
  • Immunogen

    Synthetic peptide within Human PHOX2B (N terminal). The exact sequence is proprietary. Conjugated to a protein carrier.
    Database link: Q99453

  • Positive control

    • IP: IMR32 whole cell extract. WB: IMR32, SK-N-AS and SH-SY5Y whole cell extracts.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin 300
    Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurology process
    • Neural Signal Transduction
    • Neuroscience
    • Neurology process
    • Neurogenesis
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Developmental Families
    • Other

Images

  • Immunoprecipitation - Anti-PHOX2B antibody - N-terminal (ab227719)
    Immunoprecipitation - Anti-PHOX2B antibody - N-terminal (ab227719)

    PHOX2B was immunoprecipitated from IMR32 (human neuroblast cell line) whole cell extract with 5 µg ab227719. Western blot was performed from the immunoprecipitate using ab227719. Anti-Rabbit IgG was used as a secondary reagent.

    Lane 1: Control IgG IP in IMR32 whole cell extract.

    Lane 2: ab227719 IP in IMR32 whole cell extract.

  • Western blot - Anti-PHOX2B antibody - N-terminal (ab227719)
    Western blot - Anti-PHOX2B antibody - N-terminal (ab227719)
    All lanes : Anti-PHOX2B antibody - N-terminal (ab227719) at 1/1000 dilution

    Lane 1 : IMR32 (human neuroblast cell line) whole cell extract
    Lane 2 : SK-N-AS (human neuroblastoma cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 32 kDa



    12% SDS-PAGE gel.

  • Western blot - Anti-PHOX2B antibody - N-terminal (ab227719)
    Western blot - Anti-PHOX2B antibody - N-terminal (ab227719)
    Anti-PHOX2B antibody - N-terminal (ab227719) at 1/1000 dilution + SH-SY5Y (human neuroblastoma cell line from bone marrow) whole cell extract at 30 µg

    Predicted band size: 32 kDa



    12% SDS-PAGE gel.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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