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Cell Biology Proteolysis / Ubiquitin Proteasome / Ubiquitin Ubiquitin E3 Enzymes RING Finger E3 Ligase

Anti-RNF185 antibody [EPR14070-94] - BSA and Azide free (ab250564)

Anti-RNF185 antibody [EPR14070-94] - BSA and Azide free (ab250564)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14070-94] to RNF185 + RNF5/NG2 - BSA and Azide free
  • Suitable for: IP, WB
  • Reacts with: Human

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Overview

  • Product name

    Anti-RNF185 + RNF5/NG2 antibody [EPR14070-94] - BSA and Azide free
  • Description

    Rabbit monoclonal [EPR14070-94] to RNF185 + RNF5/NG2 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab250564 is the carrier-free version of ab181999.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR14070-94
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Ubiquitin E3 Enzymes
    • RING Finger E3 Ligase
    • Immunology
    • Immune System Diseases
    • Antiviral Signaling
    • Cell Biology
    • Other Antibodies
    • Other Antibodies
    • Cancer
    • Tumor biomarkers
    • Other
    • Microbiology
    • Interspecies Interaction
    • Host Immune Response

Images

  • Immunoprecipitation - Anti-RNF185 antibody [EPR14070-94] - BSA and Azide free (ab250564)
    Immunoprecipitation - Anti-RNF185 antibody [EPR14070-94] - BSA and Azide free (ab250564)
    This data was developed using ab181999, the same antibody clone in a different buffer formulation.Western blot analysis of immunoprecipitation pellet from HeLa cell lysate immunoprecipitated using ab181999 at 1/40 dilution (lane 1) or a negative control (lane 2) followed by Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.
  • Western blot - Anti-RNF185 antibody [EPR14070-94] - BSA and Azide free (ab250564)
    Western blot - Anti-RNF185 antibody [EPR14070-94] - BSA and Azide free (ab250564)
    All lanes : Anti-RNF185 antibody [EPR14070-94] (ab181999) at 1/5000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : Human fetal brain lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L)-HRP at 1/1000 dilution

    Predicted band size: 20 kDa



    This data was developed using ab181999, the same antibody clone in a different buffer formulation.

     

  • Anti-RNF185 antibody [EPR14070-94] - BSA and Azide free (ab250564)
    Anti-RNF185 antibody [EPR14070-94] - BSA and Azide free (ab250564)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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