Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SKP2 antibody [EPR3306(2)] - BSA and Azide free (ab248010)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3306(2)] to SKP2 - BSA and Azide free
  • Suitable for: WB, ICC
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-SKP2 antibody [EPR3306(2)] - BSA and Azide free
    See all SKP2 primary antibodies

  • Description

    Rabbit monoclonal [EPR3306(2)] to SKP2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab248010 is the carrier-free version of ab124799. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab248010 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-SKP2 antibody [EPR3306(2)] - BSA and Azide free (ab248010)
    Western blot - Anti-SKP2 antibody [EPR3306(2)] - BSA and Azide free (ab248010)
    All lanes : Anti-SKP2 antibody [EPR3306(2)] (ab124799) at 1/1000 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : SKP2 knockout HAP1 whole cell lysate
    Lane 3 : HeLa whole cell lysate
    Lane 4 : MCF7 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 46, 48 kDa



    This data was developed using ab124799, the same antibody clone in a different buffer formulation.

    Lanes 1 - 4: Merged signal (red and green). Green - ab124799 observed at 52 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab124799 was shown to recognize in wild-type HAP1 cells as signal was lost at the expected MW in SKP2 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and SKP2 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% NF Milk. ab124799 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4­°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-SKP2 antibody [EPR3306(2)] - BSA and Azide free (ab248010)
    Western blot - Anti-SKP2 antibody [EPR3306(2)] - BSA and Azide free (ab248010)
    All lanes : Anti-SKP2 antibody [EPR3306(2)] (ab124799) at 1/1000 dilution

    Lane 1 : 293T cell lysates
    Lane 2 : HeLa cell lysates
    Lane 3 : SW480 cell lysates
    Lane 4 : LnCaP cell lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 46, 48 kDa



    This data was developed using ab124799, the same antibody clone in a different buffer formulation.

  • Immunocytochemistry - Anti-SKP2 antibody [EPR3306(2)] - BSA and Azide free (ab248010)
    Immunocytochemistry - Anti-SKP2 antibody [EPR3306(2)] - BSA and Azide free (ab248010)
    This data was developed using ab124799, the same antibody clone in a different buffer formulation.ab124799, at a 1/50 dilution, staining SKP2 in HeLa cells by Immunofluorescence.
  • Anti-SKP2 antibody [EPR3306(2)] - BSA and Azide free (ab248010)
    Anti-SKP2 antibody [EPR3306(2)] - BSA and Azide free (ab248010)

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