Anti-NFIB / NF1B2 antibody (ab264332)
Key features and details
- Rabbit polyclonal to NFIB / NF1B2
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-NFIB / NF1B2 antibody
See all NFIB / NF1B2 primary antibodies -
Description
Rabbit polyclonal to NFIB / NF1B2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide within Human NFIB/ NF1B2 aa 325-375. The exact sequence is proprietary. NP_005587.2
Database link: O00712 -
Positive control
- WB: HeLa whole cell lysate. IP: HeLa whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-NFIB / NF1B2 antibody (ab264332)All lanes : Anti-NFIB / NF1B2 antibody (ab264332) at 0.1 µg/ml
Lane 1 : HeLa human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 47 kDa
Exposure time: 30 seconds
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Immunoprecipitation - Anti-NFIB / NF1B2 antibody (ab264332)NFIB / NF1B2 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) using ab264332 at 6 μg per reaction. Western blot was performed on the immunoprecipitates using ab264332 at 1 μg/ml.
Lane 1: ab264332 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in Hela whole cell lysate.Exposure time: 3 seconds.
