Anti-SIK1 (phospho T182) + SIK2 (phospho T175) + SIK3 (phospho T163) antibody [EPR19196] (ab199474)
![Anti-SIK1 (phospho T182) + SIK2 (phospho T175) + SIK3 (phospho T163) antibody [EPR19196] (ab199474)](https://www.abcam.com/ps/products/199/ab199474/Images/ab199474-281286-anti-snf1lk-phospho-t182-snf1lk2-phospho-t175-sik3-phospho-t163-antibody-epr19196-western-blot.jpg "Anti-SIK1 (phospho T182) + SIK2 (phospho T175) + SIK3 (phospho T163) antibody [EPR19196] (ab199474)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19196] to SIK1 (phospho T182) + SIK2 (phospho T175) + SIK3 (phospho T163)
- Suitable for: Dot blot, WB
- Reacts with: Human
Overview
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Product name
Anti-SIK1 (phospho T182) + SIK2 (phospho T175) + SIK3 (phospho T163) antibody [EPR19196] -
Description
Rabbit monoclonal [EPR19196] to SIK1 (phospho T182) + SIK2 (phospho T175) + SIK3 (phospho T163) -
Host species
Rabbit -
Specificity
Expected to react with Snf1lk (when phosphorylated at T182) and SIK3 ((when phosphorylated at T163). -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HEK-293T transfected with human Snf1lk or Snf1lk2 or SIK3 (WT) whole cell lysate.
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General notes
This product was previously labelled as Snf1lk2, Snf1lk+Snf1lk2+SIK3
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19196 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-SIK1 (phospho T182) + SIK2 (phospho T175) + SIK3 (phospho T163) antibody [EPR19196] (ab199474)All lanes : Anti-SIK1 (phospho T182) + SIK2 (phospho T175) + SIK3 (phospho T163) antibody [EPR19196] (ab199474) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with empty vector (control), whole cell lysate
Lane 2 : HEK-293T transfected with a human SIK3 (WT) expression vector, whole cell lysate
Lane 3 : HEK-293T transfected with a human SIK3 (WT) expression vector, then treated with alkaline phosphatase for 1 hour, whole cell lysate
Lane 4 : HEK-293T transfected with a human SIK3 T163A mutant expression vector, whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Observed band size: 140 kDa why is the actual band size different from the predicted?
Exposure time: 1 minuteBlocking and dilution buffer and concentration: 5% NFDM/TBST.
The plasmids were kindly provided by our collaborator Dr. Yi Rao, Peking University.
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![Western blot - Anti-SIK1 (phospho T182) + SIK2 (phospho T175) + SIK3 (phospho T163) antibody [EPR19196] (ab199474)](https://www.abcam.com/ps/products/199/ab199474/Images/ab199474-281285-anti-snf1lk-phospho-t182-snf1lk2-phospho-t175-sik3-phospho-t163-antibody-epr19196-western-blot.jpg)
Western blot - Anti-SIK1 (phospho T182) + SIK2 (phospho T175) + SIK3 (phospho T163) antibody [EPR19196] (ab199474)All lanes : Anti-SIK1 (phospho T182) + SIK2 (phospho T175) + SIK3 (phospho T163) antibody [EPR19196] (ab199474) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with an empty vector (control), whole cell lysate
Lane 2 : HEK-293T transfected with a human SIK2 (WT) expression vector, whole cell lysate
Lane 3 : HEK-293T transfected with a human SIK2 (WT) expression vector, then treated with alkaline phosphatase for 1 hour, whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Observed band size: 104 kDa why is the actual band size different from the predicted?
Exposure time: 30 secondsBlocking and dilution buffer and concentration: 5% NFDM/TBST.
The plasmids were kindly provided by our collaborator Dr. Yi Rao, Peking University.
-
![Western blot - Anti-SIK1 (phospho T182) + SIK2 (phospho T175) + SIK3 (phospho T163) antibody [EPR19196] (ab199474)](https://www.abcam.com/ps/products/199/ab199474/Images/ab199474-281284-anti-snf1lk-phospho-t182-snf1lk2-phospho-t175-sik3-phospho-t163-antibody-epr19196-western-blot.jpg)
Western blot - Anti-SIK1 (phospho T182) + SIK2 (phospho T175) + SIK3 (phospho T163) antibody [EPR19196] (ab199474)All lanes : Anti-SIK1 (phospho T182) + SIK2 (phospho T175) + SIK3 (phospho T163) antibody [EPR19196] (ab199474) at 1/5000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with an empty vector (control), whole cell lysate
Lane 2 : HEK-293T transfected with a human SIK1 (WT) expression vector, whole cell lysate
Lane 3 : HEK-293T transfected with a human SIK1 (WT) expression vector, then treated with alkaline phosphatase for 1 hour, whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Observed band size: 85 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking and dilution buffer and concentration: 5% NFDM/TBST.
The plasmids were kindly provided by our collaborator Dr. Yi Rao, Peking University.
-
![Dot Blot - Anti-SIK1 (phospho T182) + SIK2 (phospho T175) + SIK3 (phospho T163) antibody [EPR19196] (ab199474)](https://www.abcam.com/ps/products/199/ab199474/Images/ab199474-281283-anti-snf1lk-phospho-t182-snf1lk2-phospho-t175-sik3-phospho-t163-antibody-epr19196-dot-blot.jpg)
Dot Blot - Anti-SIK1 (phospho T182) + SIK2 (phospho T175) + SIK3 (phospho T163) antibody [EPR19196] (ab199474)Dot blot analysis of Snf1lk2 (phospho T175) labeled with ab199474 at 1/1,000 dilution.
Lane 1: Snf1lk2 (phospho T175) peptide
Lane 2: Snf1lk2 non-phospho peptide
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100,000 dilution was used as secondary antibody.
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
-
![Anti-SIK1 (phospho T182) + SIK2 (phospho T175) + SIK3 (phospho T163) antibody [EPR19196] (ab199474)](https://www.abcam.com/ps/products/199/ab199474/Images/ab199474-6-benefits-of-recombinant-antibodies.png)
Anti-SIK1 (phospho T182) + SIK2 (phospho T175) + SIK3 (phospho T163) antibody [EPR19196] (ab199474)