Antibodies, Proteins, Kits and Reagents for Life Science

308 236 ₸ Product size

100 µl 308 236 ₸

Order now and get it on Wednesday March 03, 2021

Anti-SIK1 (phospho T182) + SIK2 (phospho T175) + SIK3 (phospho T163) antibody [EPR19196] (ab199474)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR19196] to SIK1 (phospho T182) + SIK2 (phospho T175) + SIK3 (phospho T163)
Suitable for: Dot blot, WB
Reacts with: Human

Product name

Anti-SIK1 (phospho T182) + SIK2 (phospho T175) + SIK3 (phospho T163) antibody [EPR19196]
Description

Rabbit monoclonal [EPR19196] to SIK1 (phospho T182) + SIK2 (phospho T175) + SIK3 (phospho T163)
Host species

Rabbit
Specificity

Expected to react with Snf1lk (when phosphorylated at T182) and SIK3 ((when phosphorylated at T163).
Tested Applications & Species

Application Species
WB
Human

See all applications and species data
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HEK-293T transfected with human Snf1lk or Snf1lk2 or SIK3 (WT) whole cell lysate.
General notes
This product was previously labelled as Snf1lk2, Snf1lk+Snf1lk2+SIK3

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR19196
Isotype

IgG
Research areas

Western blot - Anti-SIK1 (phospho T182) + SIK2 (phospho T175) + SIK3 (phospho T163) antibody [EPR19196] (ab199474)

All lanes : Anti-SIK1 (phospho T182) + SIK2 (phospho T175) + SIK3 (phospho T163) antibody [EPR19196] (ab199474) at 1/1000 dilution

Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with empty vector (control), whole cell lysate
Lane 2 : HEK-293T transfected with a human SIK3 (WT) expression vector, whole cell lysate
Lane 3 : HEK-293T transfected with a human SIK3 (WT) expression vector, then treated with alkaline phosphatase for 1 hour, whole cell lysate
Lane 4 : HEK-293T transfected with a human SIK3 T163A mutant expression vector, whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Observed band size: 140 kDa
why is the actual band size different from the predicted?

Exposure time: 1 minute

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

The plasmids were kindly provided by our collaborator Dr. Yi Rao, Peking University.
Western blot - Anti-SIK1 (phospho T182) + SIK2 (phospho T175) + SIK3 (phospho T163) antibody [EPR19196] (ab199474)

All lanes : Anti-SIK1 (phospho T182) + SIK2 (phospho T175) + SIK3 (phospho T163) antibody [EPR19196] (ab199474) at 1/1000 dilution

Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with an empty vector (control), whole cell lysate
Lane 2 : HEK-293T transfected with a human SIK2 (WT) expression vector, whole cell lysate
Lane 3 : HEK-293T transfected with a human SIK2 (WT) expression vector, then treated with alkaline phosphatase for 1 hour, whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Observed band size: 104 kDa why is the actual band size different from the predicted?

Exposure time: 30 seconds

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

The plasmids were kindly provided by our collaborator Dr. Yi Rao, Peking University.
Western blot - Anti-SIK1 (phospho T182) + SIK2 (phospho T175) + SIK3 (phospho T163) antibody [EPR19196] (ab199474)

All lanes : Anti-SIK1 (phospho T182) + SIK2 (phospho T175) + SIK3 (phospho T163) antibody [EPR19196] (ab199474) at 1/5000 dilution

Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with an empty vector (control), whole cell lysate
Lane 2 : HEK-293T transfected with a human SIK1 (WT) expression vector, whole cell lysate
Lane 3 : HEK-293T transfected with a human SIK1 (WT) expression vector, then treated with alkaline phosphatase for 1 hour, whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Observed band size: 85 kDa why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

The plasmids were kindly provided by our collaborator Dr. Yi Rao, Peking University.
Dot Blot - Anti-SIK1 (phospho T182) + SIK2 (phospho T175) + SIK3 (phospho T163) antibody [EPR19196] (ab199474)

Dot blot analysis of Snf1lk2 (phospho T175) labeled with ab199474 at 1/1,000 dilution.

Lane 1: Snf1lk2 (phospho T175) peptide

Lane 2: Snf1lk2 non-phospho peptide

Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100,000 dilution was used as secondary antibody.

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure time: 3 minutes.
Anti-SIK1 (phospho T182) + SIK2 (phospho T175) + SIK3 (phospho T163) antibody [EPR19196] (ab199474)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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