Anti-glutathione S transferase Omega 1/p28 antibody [EPR8370(B)] - BSA and Azide free (ab248304)
![Anti-glutathione S transferase Omega 1/p28 antibody [EPR8370(B)] - BSA and Azide free (ab248304)](https://www.abcam.com/ps/products/248/ab248304/Images/ab248304-410176-anti-glutathione-s-transferase-omega-1p28-antibody-epr8370-western-blot-human-knockout-lysate.jpg "Anti-glutathione S transferase Omega 1/p28 antibody [EPR8370(B)] - BSA and Azide free (ab248304)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8370(B)] to glutathione S transferase Omega 1/p28 - BSA and Azide free
- Suitable for: IHC-P, WB
- Knockout validated
- Reacts with: Rat, Human
Overview
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Product name
Anti-glutathione S transferase Omega 1/p28 antibody [EPR8370(B)] - BSA and Azide free
See all glutathione S transferase Omega 1/p28 primary antibodies -
Description
Rabbit monoclonal [EPR8370(B)] to glutathione S transferase Omega 1/p28 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HEK293T, Jurkat, HepG2 and BxPC-3 cell lysates; Human fetal liver lysate. IHC-P: Human colon tissue.
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General notes
ab248304 is the carrier-free version of ab129106 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab248304 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information. This product was previously labelled as glutathione S transferase Omega 1
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR8370(B) -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-glutathione S transferase Omega 1/p28 antibody [EPR8370(B)] - BSA and Azide free (ab248304)All lanes : Anti-glutathione S transferase Omega 1/p28 antibody [EPR8370(B)] (ab129106) at 1/1000 dilution
Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : GSTO1 knockout HEK293T cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : BxPC-3 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 28 kDa
Observed band size: 28 kDaThis data was developed using ab129106, the same antibody clone in a different buffer formulation.
Lanes 1-4: Merged signal (red and green). Green - ab129106 observed at 28 kDa. Red - loading control ab8245 observed at 36 kDa.
ab129106 Anti-glutathione S transferase Omega 1/p28 antibody [EPR8370(B)] was shown to specifically react with glutathione S transferase Omega 1/p28 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266277 (knockout cell lysate ab257982) was used. Wild-type and glutathione S transferase Omega 1/p28 knockout samples were subjected to SDS-PAGE. ab129106 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-glutathione S transferase Omega 1/p28 antibody [EPR8370(B)] - BSA and Azide free (ab248304)](https://www.abcam.com/ps/products/248/ab248304/Images/ab248304-402378-anti-glutathione-s-transferase-omega-1-p28-antibody-epr8370-b-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-glutathione S transferase Omega 1/p28 antibody [EPR8370(B)] - BSA and Azide free (ab248304)All lanes : Anti-glutathione S transferase Omega 1/p28 antibody [EPR8370(B)] (ab129106) at 1/1000 dilution
Lane 1 : Human fetal liver lysate
Lane 2 : BxPC3 lysate
Lane 3 : HepG2 lysate
Lane 4 : HeLa lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 28 kDaThis data was developed using ab129106, the same antibody clone in a different buffer formulation.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-glutathione S transferase Omega 1/p28 antibody [EPR8370(B)] - BSA and Azide free (ab248304)](https://www.abcam.com/ps/products/248/ab248304/Images/ab248304-1-anti-glutathione-s-transferase-omega-1-p28-antibody-epr8370-b-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-glutathione S transferase Omega 1/p28 antibody [EPR8370(B)] - BSA and Azide free (ab248304)This data was developed using ab129106, the same antibody clone in a different buffer formulation.ab129106, at 1/100 dilution, staining glutathione S transferase Omega 1/p28 in Paraffin-embedded Human colon tissue by Immunohistochemistry. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. -
![Anti-glutathione S transferase Omega 1/p28 antibody [EPR8370(B)] - BSA and Azide free (ab248304)](https://www.abcam.com/ps/products/248/ab248304/Images/ab248304-4-benefits-of-recombinant-antibodies.png)
Anti-glutathione S transferase Omega 1/p28 antibody [EPR8370(B)] - BSA and Azide free (ab248304)