Antibodies, Proteins, Kits and Reagents for Life Science

361 843 ₸ Product size

100 µl 361 843 ₸

Order now and get it on Tuesday March 09, 2021

Anti-Cytokeratin 10 antibody [EP1607IHCY] - Cytoskeleton Marker (ab76318)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP1607IHCY] to Cytokeratin 10 - Cytoskeleton Marker
Suitable for: ICC/IF, WB, IHC-P
Reacts with: Mouse, Rat, Human

Product name

Anti-Cytokeratin 10 antibody [EP1607IHCY] - Cytoskeleton Marker
See all Cytokeratin 10 primary antibodies
Description

Rabbit monoclonal [EP1607IHCY] to Cytokeratin 10 - Cytoskeleton Marker
Host species

Rabbit
Specificity

Some customers have successfully used ab76318 to staining Cytokeratin 10 in mouse tissue. Please see Abreviews for more details.

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Hacat and A431 cell lysates, human fetal, rat and mouse skin tissue lysates. IHC-P: Human skin and tonsil tissues and mouse skin tissue. ICC/IF: HACAT cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EP1607IHCY
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 10 antibody [EP1607IHCY] (ab76318) This image is courtesy of an abreview submitted by Carl Hobbs, King's College London, United Kingdom

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse skin tissue labelling Cytokeratin 10 with unpurified ab76318 at a 1/10000 dilution. The sections were subjected to heat mediated antigen retrieval. The sections were then blocked using 1% BSA for 10 mins at 21°C. ab76318 was incubated for 2 hours at 21°C. A biotin-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody (1/250).
See Abreview
Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 10 antibody [EP1607IHCY] (ab76318)

Immunocytochemistry/Immunofluorescence analysis of HACAT cells labelling Cytokeratin 10 with purified ab76318 at 1/150. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/500) and ab150120, an Alexa Fluor^® 594-conjugated goat anti-mouse IgG (1/500) were also used.
Control 1: primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor^® 594-conjugated goat anti-mouse IgG (1/500).
Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/500).
Western blot - Anti-Cytokeratin 10 antibody [EP1607IHCY] (ab76318)

All lanes : Anti-Cytokeratin 10 antibody [EP1607IHCY] - Cytoskeleton Marker (ab76318) at 1/10000 dilution (purified)

Lane 1 : HACAT cell lysate
Lane 2 : A431 cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 60 kDa
Observed band size: 60 kDa

Blocking and diluting buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 10 antibody [EP1607IHCY] (ab76318) This image is courtesy of an abreview submitted by Carl Hobbs, King's College London, United Kingdom

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skin tissue labelling Cytokeratin 10 with unpurified ab76318 at a 1/6000 dilution. The sections were subjected to heat mediated antigen retrieval. The sections were then blocked using 1% BSA for 10 mins at 21°C. ab76318 was incubated for 2 hours at 21°C. A biotin-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody (1/200).
See Abreview
Western blot - Anti-Cytokeratin 10 antibody [EP1607IHCY] (ab76318)

Anti-Cytokeratin 10 antibody [EP1607IHCY] - Cytoskeleton Marker (ab76318) at 1/50000 dilution (purified) + Human fetal skin tissue lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 60 kDa
Observed band size: 60 kDa

Blocking and diluting buffer: 5% NFDM/TBST.
Western blot - Anti-Cytokeratin 10 antibody [EP1607IHCY] (ab76318)

Anti-Cytokeratin 10 antibody [EP1607IHCY] - Cytoskeleton Marker (ab76318) at 1/50000 dilution (purified) + Rat skin tissue lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 60 kDa
Observed band size: 60 kDa

Blocking and diluting buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 10 antibody [EP1607IHCY] - Cytoskeleton Marker (ab76318)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse skin tissue labelling Cytokeratin 10 with purified ab76318 at 1/5000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Western blot - Anti-Cytokeratin 10 antibody [EP1607IHCY] (ab76318)

Anti-Cytokeratin 10 antibody [EP1607IHCY] - Cytoskeleton Marker (ab76318) at 1/200000 dilution (purified) + Mouse skin tissue lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 60 kDa
Observed band size: 60 kDa

Blocking and diluting buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 10 antibody [EP1607IHCY] - Cytoskeleton Marker (ab76318)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling Cytokeratin 10 with purified ab76318 at 1/5000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Western blot - Anti-Cytokeratin 10 antibody [EP1607IHCY] (ab76318)

Anti-Cytokeratin 10 antibody [EP1607IHCY] - Cytoskeleton Marker (ab76318) at 1/100000 dilution + HaCat cell lysate at 10 µg

Secondary
HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

Predicted band size: 60 kDa
Observed band size: 60 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 10 antibody [EP1607IHCY] - Cytoskeleton Marker (ab76318)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human normal skin tissue labelling Cytokeratin 10 with unpurified ab76318.

Green - CK10, red - PI.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 10 antibody [EP1607IHCY] (ab76318) This image is courtesy of an abreview submitted by Carl Hobbs, King's College London, United Kingdom

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat skin tissue labelling Cytokeratin 10 with unpurified ab76318 at a 1/10000 dilution. The sections were subjected to heat mediated antigen retrieval. The sections were then blocked using 1% BSA for 10 mins at 21°C. ab76318 was incubated for 2 hours at 21°C. A biotin-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody (1/250).
See Abreview
Anti-Cytokeratin 10 antibody [EP1607IHCY] - Cytoskeleton Marker (ab76318)

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