Antibodies, Proteins, Kits and Reagents for Life Science

Key features and details

Mouse monoclonal [OTI4E1] to MEK1
Suitable for: WB, IHC-P, ICC/IF
Reacts with: Mouse, Rat, Dog, Human, African green monkey
Isotype: IgG1

Product name

Anti-MEK1 antibody [OTI4E1]
See all MEK1 primary antibodies
Description

Mouse monoclonal [OTI4E1] to MEK1
Host species

Mouse

Tested Applications & Species

Application	Species
ICC/IF	African green monkey
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Recombinant full length protein corresponding to Human MEK1 aa 1-393.
Sequence:
MPKKKPTPIQLNPAPDGSAVNGTSSAETNLEALQKKLEELELDEQQRKRL EAFLTQKQKVGELKDDDFEK ISELGAGNGGVVFKVSHKPSGLVMARKL IHLEIKPAIRNQIIRELQVLHECNSPYIVGFYGAFYSDGEIS ICMEHM DGGSLDQVLKKAGRIPEQILGKVSIAVIKGLTYLREKHKIMHRDVKPSNI LVNSRGEIKLCDFG VSGQLIDSMANSFVGTRSYMSPERLQGTHYSVQS DIWSMGLSLVEMAVGRYPIPPPDAKELELMFGCQVE GDAAETPPRPRT PGRPLSSYGMDSRPPMAIFELLDYIVNEPPPKLPSGVFSLEFQDFVNKCL IKNPAERA DLKQLMVHAFIKRSDAEEVDFAGWLCSTIGLNQPSTPTHA AGV

Database link: NP_002746
Run BLAST with Run BLAST with
Positive control
- WB: HEK293T cells transfected with the pCMV6-ENTRY MEK1 cDNA; COS7 cells transiently transfected by pCMV6-ENTRY MEK1 cDNA; HepG2, HeLa, SVT2, A549, COS7, Jurkat, MDCK, PC12 and MCF7 cell extracts; IHC-P: Human breast adenocarcinoma, Human kidney, Human ovary Adenocarcinoma, Human pancreas, Human endometrium and Human tonsil tissues. ICC/IF: COS7 cells transiently transfected by pCMV6-ENTRY MAP2K1
General notes

The clone number has been updated from 4E1 to OTI4E1, both clone numbers name the same clone.

The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer

pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 1% BSA, PBS
Concentration information loading...
Purity

Affinity purified
Purification notes

Purified from cell culture supernatant by affinity chromatography
Clonality

Monoclonal
Clone number

OTI4E1
Isotype

IgG1
Research areas

Western blot - Anti-MEK1 antibody [OTI4E1] (ab139343)

All lanes : Anti-MEK1 antibody [OTI4E1] (ab139343) at 1/200 dilution

Lane 1 : HepG2 cell extract (Human).
Lane 2 : HeLa cell extract (Human).
Lane 3 : SVT2 cell extract (Mouse).
Lane 4 : A549 cell extract (Human)
Lane 5 : COS7 cell extract (Monkey).
Lane 6 : Jurkat cell extract (Human).
Lane 7 : MDCK cell extract (Canine).
Lane 8 : PC12 cell extract (Rat).
Lane 9 : MCF7 cell extract (Human).

Lysates/proteins at 35 µg per lane.

Predicted band size: 43 kDa
Western blot - Anti-MEK1 antibody [OTI4E1] (ab139343)

All lanes : Anti-MEK1 antibody [OTI4E1] (ab139343) at 1/200 dilution

Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control cDNA.
Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY MEK1 cDNA.

Lysates/proteins at 5 µg per lane.

Predicted band size: 43 kDa
Western blot - Anti-MEK1 antibody [OTI4E1] (ab139343)

All lanes : Anti-MEK1 antibody [OTI4E1] (ab139343) at 1/200 dilution

Lane 1 : Human testis extract
Lane 2 : Human uterus extract
Lane 3 : Human breast extract
Lane 4 : Human brain extract
Lane 5 : Human liver extract
Lane 6 : Human ovary extract
Lane 7 : Human thyroid gland extract
Lane 8 : Human colon extract

Lysates/proteins at 10 µg per lane.

Predicted band size: 43 kDa
Immunocytochemistry/ Immunofluorescence - Anti-MEK1 antibody [OTI4E1] (ab139343)

immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MEK cDNA, labelling MEK1 with ab139343 at 1/100 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEK1 antibody [OTI4E1] (ab139343)

Immunohistochemical staining of paraffin-embedded Human breast tissue adenocarcinoma labelling MEK1 with ab139343 at 1/150 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEK1 antibody [OTI4E1] (ab139343)

Immunohistochemical staining of paraffin-embedded Human Kidney tissue labelling MEK1 with ab139343 at 1/150 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEK1 antibody [OTI4E1] (ab139343)

Immunohistochemical staining of paraffin-embedded Human ovary adenocarcinoma tissue labelling MEK1 with ab139343 at 1/150 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEK1 antibody [OTI4E1] (ab139343)

Immunohistochemical staining of paraffin-embedded Human pancreas tissue labelling MEK1 with ab139343 at 1/150 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEK1 antibody [OTI4E1] (ab139343)

Immunohistochemical staining of paraffin-embedded Human endometrium tissue labelling MEK1 with ab139343 at 1/150 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEK1 antibody [OTI4E1] (ab139343)

Immunohistochemical staining of paraffin-embedded Human tonsil labelling MEK1 with ab139343 at 1/150 dilution.

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