Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-4R Tau antibody [EPR21725] (ab218314)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR21725] to 4R Tau
  • Suitable for: IP, WB, IHC-P
  • Reacts with: Mouse, Rat, Human, Recombinant fragment

Overview

  • Product name

    Anti-4R Tau antibody [EPR21725]
    See all 4R Tau primary antibodies

  • Description

    Rabbit monoclonal [EPR21725] to 4R Tau

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Mouse
    Rat
    Human
    IP
    Rat
    WB
    Mouse
    Rat
    Human
    Recombinant fragment
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human breast carcinoma and cerebrum tissue; Mouse cerebrum and colon tissue; Rat cerebral cortex and colon tissue. WB: Recombinant human 0N4R Tau, 1N4R Tau and 2N4R Tau protein; Mouse hippocampus and brain lysate; Rat hippocampus lysate. IP: Rat hippocampus lysate

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunoprecipitation - Anti-4R Tau antibody [EPR21725] (ab218314)
    Immunoprecipitation - Anti-4R Tau antibody [EPR21725] (ab218314)

    4R Tau was immunoprecipitated from 0.35 mg rat hippocampus lysate 10 µg with ab218314 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab218314 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: Rat hippocampus lysate 10 µg

    Lane 2: ab218314 IP in rat hippocampus lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab218314 in rat hippocampus lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 8 s

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-4R Tau antibody [EPR21725] (ab218314)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-4R Tau antibody [EPR21725] (ab218314)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue labeling 4R Tau with ab218314 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining on myenteric plexus of mouse colon is observed. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Western blot - Anti-4R Tau antibody [EPR21725] (ab218314)
    Western blot - Anti-4R Tau antibody [EPR21725] (ab218314)
    All lanes : Anti-4R Tau antibody [EPR21725] (ab218314) at 1/1000 dilution

    Lane 1 : Human hippocampus lysate
    Lane 2 : Mouse hippocampus lysate
    Lane 3 : Mouse brain lysate
    Lane 4 : Mouse stomach lysate
    Lane 5 : Rat hippocampus lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Lane 1 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
    Lanes 2-5 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 79 kDa



    Negative control: Mouse stomach PMID:8752131; PMID:11727254.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lanes 1-4: 3 minutes; Lanes 5: 37 seconds.

  • Western blot - Anti-4R Tau antibody [EPR21725] (ab218314)
    Western blot - Anti-4R Tau antibody [EPR21725] (ab218314)
    All lanes : Anti-4R Tau antibody [EPR21725] (ab218314) at 1/1000 dilution

    Lane 1 : His-tagged human 2N3R Tau recombinant protein (aa1-410) 10 ng
    Lane 2 : His-tagged human 2N4R Tau recombinant protein (aa1-441) 10ng
    Lane 3 : His-tagged human 0N3R Tau recombinant protein (aa1-352) 10ng
    Lane 4 : His-tagged human 0N4R Tau recombinant protein (aa1-383) 10ng
    Lane 5 : His-tagged human 1N3R Tau recombinant protein (aa1-381) 10ng
    Lane 6 : His-tagged human 1N4R Tau recombinant protein (aa1-412) 10ng

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 79 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    This antibody specifically recognizes 0N4R, 1N4R and 2N4R tau recombinant proteins. The lower bands maybe degraded tau fragments (PMID:28045602).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-4R Tau antibody [EPR21725] (ab218314)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-4R Tau antibody [EPR21725] (ab218314)

    Immunohistochemical analysis of paraffin-embedded rat colon tissue labeling 4R Tau with ab218314 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining on myenteric plexus of rat colon is observed. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-4R Tau antibody [EPR21725] (ab218314)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-4R Tau antibody [EPR21725] (ab218314)

    Immunohistochemical analysis of paraffin-embedded rat cerebral cortex tissue labeling 4R Tau with ab218314 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining on rat cerebral cortex is observed. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-4R Tau antibody [EPR21725] (ab218314)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-4R Tau antibody [EPR21725] (ab218314)

    Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling 4R Tau with ab218314 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining on mouse cerebrum (PMID: 18925637) is observed. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-4R Tau antibody [EPR21725] (ab218314)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-4R Tau antibody [EPR21725] (ab218314)

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue labeling 4R Tau with ab218314 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining on human breast carcinoma (PMID: 15914550) is observed. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-4R Tau antibody [EPR21725] (ab218314)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-4R Tau antibody [EPR21725] (ab218314)

    Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling 4R Tau with ab218314 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining in human cerebrum is observed. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Anti-4R Tau antibody [EPR21725] (ab218314)
    Anti-4R Tau antibody [EPR21725] (ab218314)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-4R Tau antibody [EPR21725] (ab218314)

© 2021 Astana Biomed Group LLP. All rights reserved.