Antibodies, Proteins, Kits and Reagents for Life Science

321 638 ₸ Product size

100 µl 321 638 ₸

Order now and get it on Tuesday March 09, 2021

Anti-4R Tau antibody [EPR21725] (ab218314)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR21725] to 4R Tau
Suitable for: IP, WB, IHC-P
Reacts with: Mouse, Rat, Human, Recombinant fragment

Product name

Anti-4R Tau antibody [EPR21725]
See all 4R Tau primary antibodies
Description

Rabbit monoclonal [EPR21725] to 4R Tau
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-P	Mouse Rat Human
IP	Rat
WB	Mouse Rat Human Recombinant fragment

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- IHC-P: Human breast carcinoma and cerebrum tissue; Mouse cerebrum and colon tissue; Rat cerebral cortex and colon tissue. WB: Recombinant human 0N4R Tau, 1N4R Tau and 2N4R Tau protein; Mouse hippocampus and brain lysate; Rat hippocampus lysate. IP: Rat hippocampus lysate
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR21725
Isotype

IgG
Research areas

Immunoprecipitation - Anti-4R Tau antibody [EPR21725] (ab218314)

4R Tau was immunoprecipitated from 0.35 mg rat hippocampus lysate 10 µg with ab218314 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab218314 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1: Rat hippocampus lysate 10 µg

Lane 2: ab218314 IP in rat hippocampus lysate

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab218314 in rat hippocampus lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 8 s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-4R Tau antibody [EPR21725] (ab218314)

Immunohistochemical analysis of paraffin-embedded mouse colon tissue labeling 4R Tau with ab218314 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining on myenteric plexus of mouse colon is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Western blot - Anti-4R Tau antibody [EPR21725] (ab218314)

All lanes : Anti-4R Tau antibody [EPR21725] (ab218314) at 1/1000 dilution

Lane 1 : Human hippocampus lysate
Lane 2 : Mouse hippocampus lysate
Lane 3 : Mouse brain lysate
Lane 4 : Mouse stomach lysate
Lane 5 : Rat hippocampus lysate

Lysates/proteins at 20 µg per lane.

Secondary
Lane 1 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
Lanes 2-5 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 79 kDa

Negative control: Mouse stomach PMID:8752131; PMID:11727254.

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure times: Lanes 1-4: 3 minutes; Lanes 5: 37 seconds.
Western blot - Anti-4R Tau antibody [EPR21725] (ab218314)

All lanes : Anti-4R Tau antibody [EPR21725] (ab218314) at 1/1000 dilution

Lane 1 : His-tagged human 2N3R Tau recombinant protein (aa1-410) 10 ng
Lane 2 : His-tagged human 2N4R Tau recombinant protein (aa1-441) 10ng
Lane 3 : His-tagged human 0N3R Tau recombinant protein (aa1-352) 10ng
Lane 4 : His-tagged human 0N4R Tau recombinant protein (aa1-383) 10ng
Lane 5 : His-tagged human 1N3R Tau recombinant protein (aa1-381) 10ng
Lane 6 : His-tagged human 1N4R Tau recombinant protein (aa1-412) 10ng

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 79 kDa

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.

This antibody specifically recognizes 0N4R, 1N4R and 2N4R tau recombinant proteins. The lower bands maybe degraded tau fragments (PMID:28045602).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-4R Tau antibody [EPR21725] (ab218314)

Immunohistochemical analysis of paraffin-embedded rat colon tissue labeling 4R Tau with ab218314 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining on myenteric plexus of rat colon is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-4R Tau antibody [EPR21725] (ab218314)

Immunohistochemical analysis of paraffin-embedded rat cerebral cortex tissue labeling 4R Tau with ab218314 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining on rat cerebral cortex is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-4R Tau antibody [EPR21725] (ab218314)

Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling 4R Tau with ab218314 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining on mouse cerebrum (PMID: 18925637) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-4R Tau antibody [EPR21725] (ab218314)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue labeling 4R Tau with ab218314 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining on human breast carcinoma (PMID: 15914550) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-4R Tau antibody [EPR21725] (ab218314)

Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling 4R Tau with ab218314 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining in human cerebrum is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Anti-4R Tau antibody [EPR21725] (ab218314)

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