Anti-Histone H3 (phospho T3, mono methyl K4) antibody (ab272138)
Key features and details
- Rabbit polyclonal to Histone H3 (phospho T3, mono methyl K4)
- Suitable for: Dot blot, ICC/IF, WB
- Reacts with: Mouse, Human, Caenorhabditis elegans
- Isotype: IgG
Overview
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Product name
Anti-Histone H3 (phospho T3, mono methyl K4) antibody
See all Histone H3 primary antibodies -
Description
Rabbit polyclonal to Histone H3 (phospho T3, mono methyl K4) -
Host species
Rabbit -
Tested applications
Suitable for: Dot blot, ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Human, Caenorhabditis elegans -
Immunogen
Synthetic peptide corresponding to Human Histone H3 (phospho T3, mono methyl K4).
Database link: Q71DI3 -
Positive control
- ICC/IF: HeLa cells. WB: HeLa histone prep, NIH/3T3 histone prep, C.elegans embryo lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 30% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (phospho T3, mono methyl K4) antibody (ab272138)
Immunofluorescence analysis of 0.5% PFA-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Anti-Histone H3 (phospho T3, mono methyl K4) (green) with ab272138 at 1/100 dilution, followed by Dylight 488 secondary antibody at 1/10000 dilution. Counterstain: Nuclei and aplpha-tubulin were co-stained with DAPI (blue) and Dylight 550 (red).
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Western blot - Anti-Histone H3 (phospho T3, mono methyl K4) antibody (ab272138)All lanes : Anti-Histone H3 (phospho T3, mono methyl K4) antibody (ab272138) at 1/1000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) histone prep
Lane 2 : NIH/3T3 (mouse embryo fibroblast cell line) histone prep
Lane 3 : C.elegans embryo lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : IRDye800™ rabbit secondary antibody at 1/10000 dilution
Predicted band size: 15 kDa
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Dot Blot - Anti-Histone H3 (phospho T3, mono methyl K4) antibody (ab272138)Dot Blot analysis of modified forms of the Histone H3 immunizing peptide using ab272138 at 1/1000 dilution, followed by HRP rabbit secondary antibody at 1/40000 dilution. Load: 1, 10, and 100 picomoles of sample.
