Anti-Histone H3 (acetyl K9) antibody (ab272150)
Key features and details
- Rabbit polyclonal to Histone H3 (acetyl K9)
- Suitable for: WB, ICC/IF, ChIP, Dot blot
- Reacts with: Mouse, Human, Caenorhabditis elegans
- Isotype: IgG
Overview
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Product name
Anti-Histone H3 (acetyl K9) antibody
See all Histone H3 primary antibodies -
Description
Rabbit polyclonal to Histone H3 (acetyl K9) -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, ChIP, Dot blotmore details -
Species reactivity
Reacts with: Mouse, Human, Caenorhabditis elegans -
Immunogen
Synthetic peptide corresponding to Human Histone H3 (acetyl K9).
Database link: Q71DI3 -
Positive control
- WB: HeLa and NIH/3T3 histone preps; C.elegans embryo lysate. ICC/IF: HeLa cells. ChIP: Chromatin prepared from HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 30% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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ChIP - Anti-Histone H3 (acetyl K9) antibody (ab272150)
Chromatin Immunoprecipitation analysis using ab272150. Chromatin from one million formaldehyde cross-linked HeLa (human epithelial cell line from cervix adenocarcinoma) cells and 2 µg of ab272150 was used to IP DNA from fixed HeLa cells alongside a no antibody (No Ab) control. DNA was measured by qRT-PCR and normalized to total input (input=1).
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Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (acetyl K9) antibody (ab272150)Immunofluorescence analysis of 0.5% PFA-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Histone H3 (acetyl K9) (green) with ab272150 at 1/100 dilution for 1 hour at room temperature, followed by FITC secondary antibody at 1/10,000 for 45 minutes at room temperature.
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Western blot - Anti-Histone H3 (acetyl K9) antibody (ab272150)Anti-Histone H3 (acetyl K9) antibody (ab272150) at 1/500 dilution + NIH/3T3 (mouse embryo fibroblast cell line) histone preps at 30 µg
Secondary
IRDye800™ rabbit secondary antibody at 1/10000 dilution
Predicted band size: 15 kDa
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Western blot - Anti-Histone H3 (acetyl K9) antibody (ab272150)Anti-Histone H3 (acetyl K9) antibody (ab272150) at 1/500 dilution + HeLa (human epithelial cell line from cervix adenocarcinoma) histone preps at 30 µg
Secondary
IRDye800™ rabbit secondary antibody at 1/10000 dilution
Predicted band size: 15 kDa
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Western blot - Anti-Histone H3 (acetyl K9) antibody (ab272150)Anti-Histone H3 (acetyl K9) antibody (ab272150) at 1/500 dilution + C.elegans embryo lysate at 30 µg
Secondary
IRDye800™ rabbit secondary antibody at 1/10000 dilution
Predicted band size: 15 kDa
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Dot Blot - Anti-Histone H3 (acetyl K9) antibody (ab272150)Dot Blot of ab272150.
Lane 1: K9.
Lane 2: K9 Me1.
Lane 3: K9 Me2.
Lane 4: K9 Me3.
Lane 5: K9ac.
Load: 1, 10, and 100 picomoles of peptide. Primary antibody ab272150 at 1/1000 for 45 minutes at 4°C. Dylight™488 rabbit secondary antibody at 1/10000 for 45 minutes at room temperature.