Fixable Cell Viability Assay Kit (Fluorometric - Green) - Cytopainter (ab176742)
Key features and details
- Assay type: Cell-based (quantitative)
- Detection method: Fluorescent
- Platform: Flow cytometer
- Sample type: Adherent cells, Suspension cells
Overview
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Product name
Fixable Cell Viability Assay Kit (Fluorometric - Green) - Cytopainter
See all Cell Viability kits -
Detection method
Fluorescent -
Sample type
Adherent cells, Suspension cells -
Assay type
Cell-based (quantitative) -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
Fixable Cell Viability Assay Kit (Fluorometric - Green) | Cytopainter (ab176742) is used to evaluate the viability of mammalian cells by flow cytometry. The fluorescent dye provided in the kit is retained in cells by reacting with cellular components. For viable cells, only the cell-surface amines are available to react with the dye while for the necrotic cells or the other cells with compromised membranes, the reactive dye reacts with cell surface amines and intracellular amines, resulting in more intense fluorescent staining. The difference in fluorescence intensity between the live and dead cell populations is ~100-500 folds and can be completely preserved after fixation. The approximate fluorescence excitation is 498 nm and emission maximium is 521 nm. The Excitation source is 488 nm.
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Notes
Related assays
Review the cell health assay guide to learn about kits to perform a cell viability assay, cytotoxicity assay and cell proliferation assay.
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Platform
Flow cytometer
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 200 tests DMSO 1 x 200µl Tracking dye Green 1 vial -
Research areas
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Relevance
Cell viability is a determination of living or dead cells, based on a total cell population. Cell viability assess healthy cells in a sample, with no distinction between dividing or quiescent cells. An increase in cell viability indicates cell growth, while a decrease in viability can generally be interpreted as the result of either toxic effects of compounds/agents or suboptimal culture conditions. -
Alternative names
- cell tracking
Images
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CytoPainter Fixable Cell Viability Assay Kit (Fluorometric - Green)
Fluorescent imaging of HeLa cells using ab176742. HeLa cells were treated and stained with Tracking Dye Green. The cells were fixed in 3.7% formaldehyde and analyzed by fluorescence microscopy.
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Detection of Jurkat cell viability by CytoPainter Fixable Cell Viability Assay Kit (Fluorometric - Green)Detection of Jurkat cell viability using Abcam's CytoPainter Fixable Cell Viability Assay Kit (Fluorometric - Green) (ab176742). Jurkat cells were treated and stained with Tracking Dye Green. The cells were fixed in 3.7% formaldehyde and analyzed by flow cytometry. Live (Blue solid peak), staurosporine treated (green line) and heat-treated (red solid peak) cells were distinguished with Ex/Em = 488 nm /520 nm (FL1). The live cell population is easily distinguished from the dead cell population, and nearly identical results were obtained using unfixed cells.
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Excitation and Emission Spectra for CytoPainter Fixable Cell Viability Assay Kit (Fluorometric - Green) (ab176742)
