Free Glycerol Assay Kit (Fluorometric, High Sensitivity) (ab174092)
Key features and details
- Assay type: Quantitative
- Detection method: Fluorescent
- Platform: Microplate reader
- Sample type: Adherent cells, Other biological fluids, Plasma, Serum, Suspension cells, Tissue
- Sensitivity: 0.04 nmol/well
Overview
-
Product name
Free Glycerol Assay Kit (Fluorometric, High Sensitivity)
See all Glycerol kits -
Detection method
Fluorescent -
Sample type
Serum, Plasma, Other biological fluids, Tissue, Adherent cells, Suspension cells -
Assay type
Quantitative -
Sensitivity
0.04 nmol/well -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
Abcam's PicoProbe Free Glycerol Assay Kit (ab174092) is simple, sensitive and easy to use tool to detect small amounts of glycerol.
This assay is suitable for measuring trace amount of glycerol in samples containing reducing substances, which may interfere with oxidase-based assays. In the assay, glycerol reacts with the enzyme mix to form an intermediate, which is subsequently oxidized with the production of fluorescence. The fluorescence intensity is directly proportional to the amount of glycerol.
This assay kit can detect glycerol amount less than 40 pmol.
Visit our FAQs page for tips and troubleshooting.
-
Notes
Glycerol is a central component for synthesis of all lipids; it acts as a backbone for triglycerides and phospholipids, which plays an important role for cell membrane’s structure. Due to its low toxicity, glycerol is widely used in pharmaceutical, food and cosmetic industries.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate reader
Properties
-
Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 100 tests Glycerol Assay Buffer WM 1 x 25ml Glycerol Developer 1 vial Glycerol Enzyme Mix (lyophilized) Green 1 vial Glycerol Standard Yellow 1 x 0.2ml PicoProbe Blue 1 x 0.4ml -
Research areas
-
Relevance
Glycerol is widely used in foods, beverages, solvents, pharmaceutical and cosmetic products, etc. There is broad interest in quantification of glycerol for research and development. -
Alternative names
- Glycerin
- Glycerine
Images
-
PicoProbe Free Glycerol Assay Kit (ab174092)
Free Glycerol measured in cell lysates showing quantity (pmol) per 1 mln cells.
Samples with the concentration of 1e7 cells/mL were used. Samples were diluted 2-6 fold.
-
Glycerol Standard CurveExample of Glycerol standard curve following assay's protocol.
-
Glycerol Standard CurveExample of Glycerol standard curve following assay's protocol.
-
Measurement of Glycerol LevelsMeasurement of glycerol levels in rat heart (5 µg protein), rat kidney (5 µg) and Jurkat cell lysate (10 µg) sample following PicoProbe Free Glycerol Assay Kit (ab174092) protocol. This is example data only.
