Free Glycerol Assay Kit (ab65337)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric/Fluorometric
- Platform: Microplate reader
- Assay time: 40 min
- Sample type: Cell culture supernatant, Other biological fluids, Plasma, Serum, Tissue Extracts
- Sensitivity: 1 µM
Overview
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Product name
Free Glycerol Assay Kit
See all Glycerol kits -
Detection method
Colorimetric/Fluorometric -
Sample type
Cell culture supernatant, Serum, Plasma, Other biological fluids, Tissue Extracts -
Assay type
Quantitative -
Sensitivity
> 1 µM -
Range
1 µM - 10000 µM -
Assay time
0h 40m -
Product overview
Glycerol Assay Kit (ab65337) provides a sensitive, easy assay to measure free glycerol concentration.
In the glycerol assay protocol, glycerol is enzymatically oxidized to generate a product which reacts with the probe to generate color (λ= 570 nm) and fluorescence (Ex/Em = 535/587 nm).
This glycerol assay kit can detect 50 pmol-10 nmol (or ~1-10000 µM range) of glycerol.
Glycerol assay protocol summary:
- add samples and standards to wells
- add reaction mix and incubate for 30 min
- analyze with a microplate reader -
Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 100 tests Glycerol Assay Buffer WM 1 x 25ml Glycerol Enzyme Mix (lyophilized) Green 1 vial Glycerol Probe (in DMSO, anhydrous) Red 1 x 200µl Glycerol Standard (100 mM) Yellow 1 x 200µl -
Research areas
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Relevance
Glycerol is widely used in foods, beverages, solvents, pharmaceutical and cosmetic products, etc. There is broad interest in quantification of glycerol for research and development. -
Alternative names
- Glycerin
- Glycerine
Images
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Functional Studies - Free Glycerol Assay Kit (ab65337)
Standard curve: mean of duplicates (+/- SD) with background reads subtracted
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Functional Studies - Free Glycerol Assay Kit (ab65337)Standard curve: mean of duplicates (+/- SD) with background reads subtracted
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Functional Studies - Free Glycerol Assay Kit (ab65337)Free Glycerol measured in cell culture lysates showing quantity (nmol) per 1 mln cells.
Samples with the concentration of 1e7 cells/mL were used. Samples were diluted 2-8 fold and measured fluorometrically.
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Functional Studies - Free Glycerol Assay Kit (ab65337)Free Glycerol measured in biological fluids showing quantity (nmol) per mL of tested sample. Samples were diluted 2-8 fold and measured colorimetrically.
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Free Glycerol Assay Kit - Functional Studiesa. Measurement of free glycerol in pooled human serum (10 μL). b. Measurement of free glycerol in rat liver lysate (500 μg).
