Fixable Cell Viability Assay Kit (Fluorometric - Orange Ex 405 nm) - Cytopainter (ab176740)
Key features and details
- Assay type: Cell-based (quantitative)
- Detection method: Fluorescent
- Platform: Flow cytometer
- Sample type: Adherent cells, Suspension cells
Overview
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Product name
Fixable Cell Viability Assay Kit (Fluorometric - Orange Ex 405 nm) - Cytopainter
See all Cell Viability kits -
Detection method
Fluorescent -
Sample type
Adherent cells, Suspension cells -
Assay type
Cell-based (quantitative) -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
Abcam's Fixable Cell Viability Assay Kit (Fluorometric - Orange Ex 405 nm) | Cytopainter (ab176740) is used to evaluate the viability of mammalian cells by flow cytometry.
This particular kit is designed to uniformly label fixed mammalian cells in blue fluorescence for flow cytometry applications with violet laser excitation. The kit uses a proprietary blue fluorescent dye that is more fluorescent upon bonding to cellular components. The kit provides all the essential components with an optimized cell-labeling protocol. It is an excellent tool for preserving of fluorescent images of particular cells, and can also be used for fluorescence flow cytometry applications.
The dye is designed to label cells at Ex/Em = 398/550 nm excited with a violet laser at 405 nm.
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Notes
Related assays
Review the cell health assay guide to learn about kits to perform a cell viability assay, cytotoxicity assay and cell proliferation assay.
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Platform
Flow cytometer
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 200 tests DMSO 1 x 200µl Tracking dye Violet 550 1 vial -
Research areas
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Relevance
Cell viability is a determination of living or dead cells, based on a total cell population. Cell viability assess healthy cells in a sample, with no distinction between dividing or quiescent cells. An increase in cell viability indicates cell growth, while a decrease in viability can generally be interpreted as the result of either toxic effects of compounds/agents or suboptimal culture conditions. -
Alternative names
- cell tracking
Images
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HeLa cells stained with CytoPainter Fixable Cell Viability Assay (Fluorometric-Orange Ex 405 nm) (ab176740)
HeLa cells fixed with formaldehyde and stained with Abcam's CytoPainter Fixable Cell Viability Assay Kit (Fluorometric - Orange Ex 405 nm) (ab176740) in a Costar black wall/clear bottom 96-well plate.
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Excitation and Emission spectra for CytoPainter Fixable Cell Viability Assay Kit (Fluorometric - Orange Ex 405 nm) (ab176740)Spectrum for ab176740.
