Immunoprecipitation kit (ab206996)
Key features and details
- Sample type: Adherent cells, Suspension cells, Tissue
Overview
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Product name
Immunoprecipitation kit -
Sample type
Tissue, Adherent cells, Suspension cells -
Product overview
Abcam’s Immunoprecipitation Kit (ab206996) can be used to perform immunoprecipitation (IP) and Co-IP for functional studies of immunoprecipitated proteins/complexes and SDS-PAGE or western blot analysis of immunoprecipitated proteins and complexes.
Abcam’s Immunoprecipitation Kit provides optimized buffers for preparing cell/tissue extracts, antigen binding and washing steps. The Protein A/G Sepharose® beads provided in the kit have a higher binding capacity with broader antibody isotype binding than traditional Protein A or Protein G resins.
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Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Tested applications
Suitable for: IPmore details
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 25 tests 10X Wash Buffer 1 x 20ml Lysis Buffer (non-denaturing) 1 x 40ml Protease Inhibitor Cocktail (lyophilized) 1 vial Protein A/G Sepharose® 1 x 1ml RIPA Lysis Buffer 1 x 40ml
Images
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Functional Studies - Immunoprecipitation kit (ab206996) Lunova, Mariia et al., Scientific reportsvol. 7,1 16049., Fig 8, doi:10.1038/s41598-017-16447-6
Huh7 cultured with Si-OH, Si-NH2, or PS-NH2 NPs (all 50 µg/ml) bearing BSA or RNase (both 50 µM) as hard protein corona or bare NPs for 4 h. After 4 h post NP treatment, cells were lysed with lysis buffer from immunoprecipitation kit (Abcam). RagC-mTOR complexes were co-immunoprecipitated from the precleared cell lysates with appropriate Ab as described in the manufacturer's instruction. The resulting protein complex was eluted from the beads with Laemmli protein sample buffer for SDS-PAGE and resolved on SDS-PAGE with specific antibody against mTOR
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Comparison of Binding Capacity of Protein-A and Protein-A/G Beads.Comparison of immunoprecipitation using Protein A beads and Immunoprecipitation kit ab206996. HDAC2 activity assay of the antigen-antibody complex captured using Protein A beads or ab206996 by following the same protocol demonstrates that ab206996 is more efficient in IP than Protein A beads.
