Antibodies, Proteins, Kits and Reagents for Life Science

Anti-ERCC1 antibody [SP68] - BSA and Azide free (ab236442)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [SP68] to ERCC1 - BSA and Azide free
Suitable for: IHC-P
Reacts with: Human

Product name

Anti-ERCC1 antibody [SP68] - BSA and Azide free
See all ERCC1 primary antibodies
Description

Rabbit monoclonal [SP68] to ERCC1 - BSA and Azide free
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Human

See all applications and species data
Immunogen

Recombinant full length protein corresponding to Human ERCC1.
Database link: P07992
Positive control
- IHC-P: Human lung carcinoma, bladder transitional cell carcinoma, testis, breast ductal carcinoma, colon, colon carcinoma, hepatocellular carcinoma, lung carcinoma, prostate carcinoma, renal cell carcinoma, skin squamous cell carcinoma, Mouse colon, and Rat colon tissue.
General notes
FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Ab236442 is the carrier-free version of ab225721. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab236442 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.

Application	Species
IHC-P	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer

pH: 7.20
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Purity

Protein A/G purified
Purification notes

Purified from TCS by protein A/G.
Clonality

Monoclonal
Clone number

SP68
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERCC1 antibody [SP68] - BSA and Azide free (ab236442)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat colon tissue sections labeling ERCC1 with Purified ab225721 at 1/400 dilution (0.132 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 20mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225721)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERCC1 antibody [SP68] - BSA and Azide free (ab236442)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse colon tissue sections labeling ERCC1 with Purified ab225721 at 1/400 dilution (0.132 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 20mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225721)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERCC1 antibody [SP68] - BSA and Azide free (ab236442)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human lung carcinoma tissue sections labeling ERCC1 with Purified ab225721 at 1/100 dilution (0.53 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 20mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225721)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERCC1 antibody [SP68] - BSA and Azide free (ab236442)

Formalin-fixed, paraffin-embedded human lung carcinoma tissue stained for ERCC1 using ab225721 at 1/100 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225721).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERCC1 antibody [SP68] - BSA and Azide free (ab236442)

Formalin-fixed, paraffin-embedded human testis tissue stained for ERCC1 using ab225721 at 1/100 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225721).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERCC1 antibody [SP68] - BSA and Azide free (ab236442)

Formalin-fixed, paraffin-embedded human breast ductal carcinoma tissue stained for ERCC1 using ab225721 at 1/100 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225721).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERCC1 antibody [SP68] - BSA and Azide free (ab236442)

Formalin-fixed, paraffin-embedded human colon tissue stained for ERCC1 using ab225721 at 1/100 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225721).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERCC1 antibody [SP68] - BSA and Azide free (ab236442)

Formalin-fixed, paraffin-embedded human colon adenocarcinoma tissue stained for ERCC1 using ab225721 at 1/100 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225721).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERCC1 antibody [SP68] - BSA and Azide free (ab236442)

Formalin-fixed, paraffin-embedded human hepatocellular carcinoma tissue stained for ERCC1 using ab225721 at 1/100 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225721).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERCC1 antibody [SP68] - BSA and Azide free (ab236442)

Formalin-fixed, paraffin-embedded human lung adenocarcinoma tissue stained for ERCC1 using ab225721 at 1/100 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225721).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERCC1 antibody [SP68] - BSA and Azide free (ab236442)

Formalin-fixed, paraffin-embedded human prostate adenocarcinoma tissue stained for ERCC1 using ab225721 at 1/100 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225721).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERCC1 antibody [SP68] - BSA and Azide free (ab236442)

Formalin-fixed, paraffin-embedded human renal cell carcinoma tissue stained for ERCC1 using ab225721 at 1/100 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225721).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERCC1 antibody [SP68] - BSA and Azide free (ab236442)

Formalin-fixed, paraffin-embedded human skin squamous cell carcinoma tissue stained for ERCC1 using ab225721 at 1/100 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225721).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERCC1 antibody [SP68] - BSA and Azide free (ab236442)

Formalin-fixed, paraffin-embedded human bladder transitional cell carcinoma tissue stained for ERCC1 using ab225721 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab225721).
Anti-ERCC1 antibody [SP68] - BSA and Azide free (ab236442)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com