Anti-MDM2 antibody (ab260074)
Key features and details
- Rabbit polyclonal to MDM2
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-MDM2 antibody
See all MDM2 primary antibodies -
Description
Rabbit polyclonal to MDM2 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human MDM2 aa 100-200. The exact sequence is proprietary.
Database link: Q00987 -
Positive control
- WB: Mouse and rat brain lysate. IHC-P: Human stomach cancer tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MDM2 antibody (ab260074)
Formalin-fixed, paraffin-embedded human stomach cancer tissue labeling MDM2 with ab260074 at a 1/50 dilution (30 mins at RT) in immunohistochemistry. Counterstain: Hematoxylin. HRP-DAB Detection.
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Western blot - Anti-MDM2 antibody (ab260074)All lanes : Anti-MDM2 antibody (ab260074) at 1/1000 dilution (2 hours at RT)
Lane 1 : Mouse brain lysate
Lane 2 : Rat brain lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG: HRP, 1 hour at RT at 1/5000 dilution
Developed using the ECL technique.
Predicted band size: 55 kDa
Additional bands at: 50 kDa (possible isoform)
Exposure time: 5 minutesBlock: 5% skimmed milk in 1X TBST.
