Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MEK5 antibody [EP648Y] (ab45146)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP648Y] to MEK5
  • Suitable for: WB, ICC/IF, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-MEK5 antibody [EP648Y]
    See all MEK5 primary antibodies

  • Description

    Rabbit monoclonal [EP648Y] to MEK5

  • Host species

    Rabbit

  • Specificity

    The immunogen used for this product shares 89% homology with MAP2K4/MEK4. Cross-reactivity with this protein has not been confirmed experimentally.

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human MEK5. The exact sequence is proprietary.

  • Positive control

    • WB: HeLa whole cell lysate. ICC/IF: JAR cells. Flow Cyt: HeLa cells.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-MEK5 antibody [EP648Y] (ab45146)
    Western blot - Anti-MEK5 antibody [EP648Y] (ab45146)
    Anti-MEK5 antibody [EP648Y] (ab45146) at 1/1000 dilution (purified) + HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 49 kDa
    Observed band size: 49 kDa



    Blocking and diluting buffer: 5% NFDM/TBST

  • Immunocytochemistry/ Immunofluorescence - Anti-MEK5 antibody [EP648Y] (ab45146)
    Immunocytochemistry/ Immunofluorescence - Anti-MEK5 antibody [EP648Y] (ab45146)

    Immunocytochemistry/ Immunofluorescence analysis of JAR (Human placenta choriocarcinoma epithelial cell) cells labeling MEK5 with Purified ab45146 at 1:250 dilution (8.1μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 μg/ml). ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Flow Cytometry - Anti-MEK5 antibody [EP648Y] (ab45146)
    Flow Cytometry - Anti-MEK5 antibody [EP648Y] (ab45146)

    Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling MEK5 with purified ab45146 at 1:200 dilution (10 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

  • Immunocytochemistry/ Immunofluorescence - Anti-MEK5 antibody [EP648Y] (ab45146)
    Immunocytochemistry/ Immunofluorescence - Anti-MEK5 antibody [EP648Y] (ab45146)

    Immunofluorescent staining of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells using unpurified ab45146.

  • Western blot - Anti-MEK5 antibody [EP648Y] (ab45146)
    Western blot - Anti-MEK5 antibody [EP648Y] (ab45146)
    Anti-MEK5 antibody [EP648Y] (ab45146) at 1/10000 dilution (unpurified) + HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate

    Predicted band size: 49 kDa
    Observed band size: 49 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-MEK5 antibody [EP648Y] (ab45146)
    Immunocytochemistry/ Immunofluorescence - Anti-MEK5 antibody [EP648Y] (ab45146) This image is courtesy of an anonymous Abreview

    Unpurified ab45146 at 1/100 staining HeLa (Human epithelial cell line from cervix adenocarcinoma) cells by ICC/IF.

    The cells were paraformaldehyde fixed and blocked with goat serum prior to incubation with the antibody for 45 minutes. An Alexa Fluor® 488 conjugated goat polyclonal antibody was used as the secondary. This antibody detects MEK5 in HeLa cells as a cytoplasmic protein.

    See Abreview

  • Anti-MEK5 antibody [EP648Y] (ab45146)
    Anti-MEK5 antibody [EP648Y] (ab45146)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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