Human ICAM5 ELISA Kit (ab267625)
Key features and details
- Sensitivity: 1 ng/ml
- Range: 0.819 ng/ml - 200 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human ICAM5 ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
1 ng/ml -
Range
0.819 ng/ml - 200 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 114.9 104% - 123% Plasma 83.53 75% - 90% Cell culture media 94.65 90% - 98% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human ICAM5 ELISA Kit is designed for the quantitative determination of ICAM5 in cell culture supernatants, plasma and serum samples.
This assay employs an antibody specific for human ICAM5 coated on a 96-well plate. Standards and samples are pipetted into the wells and ICAM5 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human ICAM5 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of ICAM5 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml Anti-Human ICAM5 coated Microplate (12 x 8 wells) 1 unit Assay Diluent A 1 x 30ml Biotinylated Anti-Human ICAM5 Detection Antibody 2 vials Human ICAM5 Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
Research areas
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Relevance
ICAM5 is a member of the intercellular adhesion molecule (ICAM) family. All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin-like C2-type domains, and bind to the leukocyte adhesion LFA-1 protein. This protein is expressed on the surface of telencephalic neurons and displays two types of adhesion activity, homophilic binding between neurons and heterophilic binding between neurons and leukocytes. It may be a critical component in neuron-microglial cell interactions in the course of normal development or as part of neurodegenerative diseases. -
Cellular localization
Membrane; single-pass type I membrane protein -
Alternative names
- ICAM5 precursor
- Intercellular adhesion molecule 5
- Intercellular adhesion molecule 5 precursor
see all -
Database links
- Entrez Gene: 7087 Human
- Omim: 601852 Human
- SwissProt: Q9UMF0 Human
- Unigene: 465862 Human
Images
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Example data
These standard curves are for demonstration only. A standard curve must be run with each assay.