Antibodies, Proteins, Kits and Reagents for Life Science

301 536 ₸ Product size

100 µl 301 536 ₸

Order now and get it on Thursday March 04, 2021

Anti-liver FABP antibody [2G4] (ab82157)

Key features and details

Mouse monoclonal [2G4] to liver FABP
Suitable for: Flow Cyt, WB, ICC/IF
Reacts with: Human, Recombinant fragment
Isotype: IgG1

Product name

Anti-liver FABP antibody [2G4]
See all liver FABP primary antibodies
Description

Mouse monoclonal [2G4] to liver FABP
Host species

Mouse

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
WB	Recombinant fragment

See all applications and species data

Immunogen

Recombinant full length Human liver FABP (aa1-127) purified from E. coli (NP_001434)
Positive control
- WB: HepG2 whole cell lysate (ab7900), FABP1 Recombinant protein, Liver tissues lysate. ICC/IF: Hep3B cells. Flow Cyt: Hep3B cells.
General notes

Isotype: Mouse IgG1 heavy chain and kappa light chain

This product was changed from ascites to tissue culture supernatant on 24/01/2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage buffer

pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 10% Glycerol
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

2G4
Isotype

IgG1
Light chain type

kappa
Research areas

Immunocytochemistry/ Immunofluorescence - Anti-liver FABP antibody [2G4] (ab82157)

Immunocytochemistry/ Immunofluorescence analysis of FABP1 in Hep3B cells. The cell was stained with ab82157 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
Flow Cytometry - Anti-liver FABP antibody [2G4] (ab82157)

Flow cytometry analysis of FABP1 in Hep3B cells. The cell was stained with ab82157 at 2-5µg for 1x10⁶cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (blue), cells without incubation with primary and secondary antibody was used as the negative control (black).
Western blot - Anti-liver FABP antibody [2G4] (ab82157)

All lanes : Anti-liver FABP antibody [2G4] (ab82157) at 1/1000 dilution

Lane 1 : FABP1 Recombinant protein
Lane 2 : HepG2 cell lysate at 40 µg
Lane 3 : Liver tissues lysate at 40 µg

Secondary
All lanes : goat anti-mouse secondary antibody conjugated to HRP

Predicted band size: 14 kDa
Western blot - Anti-liver FABP antibody [2G4] (ab82157)

Anti-liver FABP antibody [2G4] (ab82157) at 1/1000 dilution + HepG2 cell lysate at 30 µg

Secondary
goat anti-mouse HRP

Developed using the ECL technique.

Predicted band size: 14 kDa
Observed band size: 14 kDa

This image was produced using the ascites version of this antibody.
Flow Cytometry - Anti-liver FABP antibody [2G4] (ab82157)

Overlay histogram showing HepG2 cells stained with ab82157 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab82157, 1µg/1x10⁶ cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.

This image was produced using the ascites version of this antibody.
Immunocytochemistry/ Immunofluorescence - Anti-liver FABP antibody [2G4] (ab82157)

Ab82157 staining liver FABP in Hep3B cells by ICC/IF (Immunocytochemistry/Immunofluorescence). Samples were incubated with primary antibody at 1:100 dilution. An Alexa Fluor^® 488 goat anti-mouse IgG was used as the secondary antibody. DAPI was used as a nuclear counterstain.

This image was produced using the ascites version of this antibody.

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