Anti-HSD17B4 antibody (ab226220)
Key features and details
- Rabbit polyclonal to HSD17B4
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-HSD17B4 antibody
See all HSD17B4 primary antibodies -
Description
Rabbit polyclonal to HSD17B4 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP Human
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Immunogen
Synthetic peptide within Human HSD17B4 aa 600-650. The exact sequence is proprietary.
Database link: P51659 -
Positive control
- IP: HEK-293T whole cell lysate.
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General notes
ab226220 has not performed satisfactorily when used for WB of HSD17B4 in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of HSD17B4.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab226220 was affinity purified using an epitope specific to HSD17B4 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-HSD17B4 antibody (ab226220)
HSD17B4 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (prepared using NETN lysis buffer; 1 mg for IP, 20% of IP loaded) with ab226220 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226220 at 0.4 µg/ml.
Lane 1: ab226220 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds.
