DOK3 overexpression 293T lysate (whole cell) (ab94169)
Overview
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Product name
DOK3 overexpression 293T lysate (whole cell) -
General notes
ab94169 is a 293T cell transfected lysate in which Human DOK3 has been transiently over-expressed using a pCMV-DOK3 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more detailed about how the transfected lysate was prepared view preparation notes
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Tested applications
Suitable for: WBmore details
Properties
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Mycoplasma free
Yes -
Form
Liquid -
Storage instructions
Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles. -
Storage buffer
Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Research areas
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Background
Function: DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK3 is a negative regulator of JNK signaling in B-cells through interaction with INPP5D/SHIP1. May modulate Abl function. Tissue specificity: Expressed in spleen. Similarity: Belongs to the DOK family. Type A subfamily. Contains 1 IRS-type PTB domain. Contains 1 PH domain. Domain: PTB domain mediates receptor interaction. PTM: Constitutively tyrosine-phosphorylated. On IL2 stimulation, phosphorylated on C-terminal tyrosine residues possibly by Src kinases. Can also be phosphorylated by ABL kinase.
Images
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SDS-PAGE - DOK3 overexpression 293T lysate (whole cell) (ab94169)ab94169 at 15μg/lane on an SDS-PAGE gel.
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Western blot - DOK3 overexpression 293T lysate (whole cell) (ab94169)All lanes : Anti-DOK3 antibody (ab89327) at 1/500 dilution
Lane 1 :DOK3 overexpression 293T lysate (whole cell) (ab94169)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugate at 1/2500 dilution