Rat FSTL1 ELISA Kit (ab246546)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 24 pg/ml
- Range: 78 pg/ml - 5000 pg/ml
- Sample type: Cit plasma, EDTA Plasma, Hep Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Rat
Overview
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Product name
Rat FSTL1 ELISA Kit
See all FSTL1/FRP kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 3.6% Inter-assay Sample n Mean SD CV% Serum 3 5.2% -
Sample type
Urine, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
24 pg/ml -
Range
78 pg/ml - 5000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Urine 110 107% - 113% Serum 101 99% - 103% Hep Plasma 92 84% - 98% EDTA Plasma 96 91% - 105% Cit plasma 100 90% - 107% -
Assay duration
One step assay -
Species reactivity
Reacts with: Rat
Does not react with: Cow -
Product overview
Rat FSTL1 ELISA Kit (ab246546) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of FSTL1 protein in cit plasma, edta plasma, hep plasma, serum, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Rat FSTL1 with 24 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Rat FSTL1 Capture Antibody 1 x 600µl 10X Rat FSTL1 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BR 1 x 6ml Plate Seals 1 unit Rat FSTL1 Lyophilized recombinant Protein 2 vials Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
May modulate the action of some growth factors on cell proliferation and differentiation. Binds heparin. -
Tissue specificity
Overexpressed in synovial tissues from rheumatoid arthritis. -
Sequence similarities
Contains 2 EF-hand domains.
Contains 1 follistatin-like domain.
Contains 1 Kazal-like domain.
Contains 1 VWFC domain. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Anti FSTL1
- FLJ50214
- FLJ52277
see all -
Database links
- Entrez Gene: 79210 Rat
- SwissProt: Q62632 Rat
- Unigene: 95652 Rat
Images
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Other - Rat FSTL1 ELISA Kit (ab246546)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of rat FSTL1 standard curve in Sample Diluent NS.The FSTL1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native FSTL1 in rat serum and plasmas samples.The concentrations of FSTL1 were measured in duplicates, interpolated from the FSTL1 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 5%, plasma (EDTA) 5%, plasma (citrate) 2.5%, plasma (heparin) 2.5% and urine 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean FSTL1 concentration was determined to be 36,218 pg/mL in serum, 72,246 pg/mL in plasma (EDTA), 58,994 pg/mL in plasma (citrate), and 75,205 pg/mL in plasma (heparin).
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Interpolated concentrations of native FSTL1 in rat urine samples.The concentrations of FSTL1 were measured in duplicates, interpolated from the FSTL1 standard curves and corrected for sample dilution. Undiluted samples are as follows: urine 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean FSTL1 concentration was determined to be 4,662 pg/mL in urine.
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Sandwich ELISA - Rat FSTL1 ELISA Kit (ab246546)To learn more about the advantages of recombinant antibodies see here.
