Human Apolipoprotein A-IV Matched Antibody Pair Kit (ab220140)
Key features and details
- Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
- For economical ELISA and ELISA-based assay development
- Reacts with: Human
- Range: 250 pg/ml - 16000 pg/ml
Overview
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Product name
Human Apolipoprotein A-IV Matched Antibody Pair Kit
See all APOA4/Apo-AIV kits -
Detection method
Colorimetric -
Assay type
ELISA set -
Sensitivity
3.74 pg/ml -
Range
250 pg/ml - 16000 pg/ml -
Species reactivity
Reacts with: Human -
Product overview
Human Apolipoprotein A-IV Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant human Apolipoprotein A-IV.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab214567. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
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Tested applications
Suitable for: ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 10 x 96 tests 5 x 96 tests Human Apolipoprotein A-IV Capture Antibody 2 x 50µg 1 x 50µg Human Apolipoprotein A-IV Detector Antibody 2 x 12.5µg 1 x 12.5µg Human Apolipoprotein A-IV Lyophilized Protein 2 vials 1 vial -
Research areas
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Relevance
APOA4 (apolipoprotein A-IV) is a component of HDL and chylomicrons. Its primary site of synthesis is the intestine, in association with lymph chylomicron particles. Although its precise function is not known, APOA4 is a potent activator of lecithin-cholesterol acyltransferase (LCAT) in vitro. In rodents, Apo A-IV inhibits gastric emptying and serves as a satiety factor whose synthesis and secretion are increased by the ingestion of dietary fat. It also possesses anti-inflammatory and antiatherogenic properties -
Cellular localization
Secreted -
Alternative names
- Apo AIV
- APOA 4
- ApoA IV
see all -
Database links
- Entrez Gene: 337 Human
- Omim: 107690 Human
- SwissProt: P06727 Human
- Unigene: 1247 Human
Images
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Human Apolipoprotein A-IV standard curve.
Standard calibration curve. Background subtracted values are graphed.
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Sandwich ELISA - Human Apolipoprotein A-IV Matched Antibody Pair Kit (ab220140)To learn more about the advantages of recombinant antibodies see here.
