Human Apolipoprotein A-IV ELISA Kit, Fluorescent (ab229428)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 10.8 pg/ml
- Range: 39.1 pg/ml - 160000 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Saliva, Serum, Tissue Extracts, Urine
- Detection method: Fluorescent
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
-
Product name
Human Apolipoprotein A-IV ELISA Kit, Fluorescent
See all APOA4/Apo-AIV kits -
Detection method
Fluorescent -
Precision
Intra-assay Sample n Mean SD CV% Human serum 8 3.8% Inter-assay Sample n Mean SD CV% Human serum 3 6.4% -
Sample type
Cell culture supernatant, Saliva, Urine, Serum, Tissue Extracts, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
10.8 pg/ml -
Range
39.1 pg/ml - 160000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Saliva 78 76% - 79% Urine 97 95% - 98% Serum 98 90% - 101% Tissue Extracts 103 102% - 105% Cell culture media 103 102% - 103% Hep Plasma 98 95% - 101% EDTA Plasma 104 102% - 107% Cit plasma 91 90% - 94% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human
Does not react with: Cow -
Product overview
Apolipoprotein A-IV in vitro CatchPoint® SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Apolipoprotein A-IV protein in human serum, plasma, saliva, urine, cell culture supernatants, and tissue extract samples.
This CatchPoint SimpleStep ELISA kit has been optimized for Molecular Devices Microplate Readers. Click here for a list of recommended Microplate Readers.
If using a Molecular Devices’ plate reader supported by SoftMax® Pro software, a preconfigured protocol for these CatchPoint SimpleStep ELISA Kits is available with all the protocol and analysis settings at www.softmaxpro.org.The CatchPoint® SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. CatchPoint® HRP Development Solution containing the Stoplight Red Substrate is added. During incubation, the substrate is catalyzed by HRP generating a fluorescent product. Signal is generated proportionally to the amount of bound analyte and the intensity is measured in a fluorescence plater reader at 530/570/590 nm Excitation/Cutoff/Emission.
-
Notes
Human Apolipoprotein A-IV is a 45kDa glycoprotein with activities involving lipid transport. Specifically, Apolipoprotein A-IV is a component of high density lipoprotein (HDL) and chylomicrons. Its primarily synthesized is the intestine in association with lymph chylomicron particles. Although its precise function is not known, Apolipoprotein A-IV is a potent activator of lecithin-cholesterol acyltransferase (LCAT) in vitro. Human Apolipoprotein A-IV consists of a 20 amino acid (aa) signal peptide and 376 aa mature chain. Human Apolipoprotein A-IV is 62% identical to both mouse and rat Apolipoprotein A-IV.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
-
Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 100X Stoplight Red Substrate 1 x 120µl 10X Human Apolipoprotein A-IV Capture Antibody 1 x 600µl 10X Human Apolipoprotein A-IV Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 500X Hydrogen Peroxide (H2O2, 3%) 1 x 50µl 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 5BI 1 x 6ml Human Apolipoprotein A-IV Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated Black 96-Well Microplate 1 unit Stoplight Red Substrate Buffer 1 x 12ml -
Research areas
-
Relevance
APOA4 (apolipoprotein A-IV) is a component of HDL and chylomicrons. Its primary site of synthesis is the intestine, in association with lymph chylomicron particles. Although its precise function is not known, APOA4 is a potent activator of lecithin-cholesterol acyltransferase (LCAT) in vitro. In rodents, Apo A-IV inhibits gastric emptying and serves as a satiety factor whose synthesis and secretion are increased by the ingestion of dietary fat. It also possesses anti-inflammatory and antiatherogenic properties -
Cellular localization
Secreted -
Alternative names
- Apo AIV
- APOA 4
- ApoA IV
see all -
Database links
- Entrez Gene: 337 Human
- Omim: 107690 Human
- SwissProt: P06727 Human
- Unigene: 1247 Human
Images
-
Other - Human Apolipoprotein A-IV ELISA Kit, Fluorescent (ab229428)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
-
Example of human Apolipoprotein A-IV standard curve in Sample Diluent NS.The Apolipoprotein A-IV standard curve was prepared as described in Section 10. Background-subtracted data values (mean +/- SD) are graphed.
-
Interpolated concentrations of native Apolipoprotein A-IV in human serum and plasma samples.The concentrations of Apolipoprotein A-IV were measured in duplicates, interpolated from the Apolipoprotein A-IV standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:32,000, plasma (citrate) 1:16,000, plasma (EDTA) 1:16,000, and plasma (heparin) 1:16000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Apolipoprotein A-IV concentration was determined to be 71.65 µg/mL in neat serum, 48.04 µg/mL in neat plasma (citrate), 42.94 µg/mL in neat plasma (EDTA) and 51.18 µg/mL in neat plasma (heparin).
-
Interpolated concentrations of native Apolipoprotein A-IV in human placenta and human liver homogenate tissue extracts based on 500 µg/mL and 400 µg/mL extract loads, respectively.The concentrations of Apolipoprotein A-IV were measured in duplicate and interpolated from the Apolipoprotein A-IV standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Apolipoprotein A-IV concentration was determined to be 4,829 pg/mL in placenta extract and 17,016 pg/mL in liver homogenate extract.
-
Interpolated concentrations of spiked Apolipoprotein A-IV in human saliva and urine samples.The concentrations of Apolipoprotein A-IV were measured in duplicates, interpolated from the Apolipoprotein A-IV standard curves and corrected for sample dilution. Undiluted samples are as follows: saliva 6.25% and urine 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Apolipoprotein A-IV concentration was determined to be 177,941 pg/mL in neat saliva (spiked) and 80,680 pg/mL in neat urine (spiked).
-
Interpolated concentrations of native Apolipoprotein A-IV in human saliva and urine samples.Saliva was analyzed at 6.25% and urine at 12.5%. The concentrations of Apolipoprotein A-IV were measured in duplicates, interpolated from the Apolipoprotein A-IV standard curves and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Apolipoprotein A-IV concentration was determined to be 25,370 pg/mL in neat saliva (native) and 3903 pg/mL in neat urine (native).
-
Serum from ten individual healthy human female donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean human Apolipoprotein A-IV concentration was determined to be 192.8 µg/mL with a range of 9.2 – 418.0 µg/mL.
-
Sandwich ELISA - Human Apolipoprotein A-IV ELISA Kit, Fluorescent (ab229428)To learn more about the advantages of recombinant antibodies see here.
