Anti-FATP1 antibody (ab81875)
Key features and details
- Rabbit polyclonal to FATP1
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-FATP1 antibody
See all FATP1 primary antibodies -
Description
Rabbit polyclonal to FATP1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human FATP1 aa 250-350 conjugated to keyhole limpet haemocyanin.
(Peptide available asab104213) -
Positive control
- This antibody gave a positive signal in Human Skeletal Muscle and Human Liver Tissue Lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab81875 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species ICC/IF HumanWB HumanAll applications DogApplication Abreviews Notes ICC/IF Use a concentration of 1 µg/ml.WB Use a concentration of 1 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 71 kDa).Notes ICC/IF
Use a concentration of 1 µg/ml.WB
Use a concentration of 1 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 71 kDa).Target
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Relevance
SLC27A1 (Solute carrier family 27 member 1) is involved in translocation of long-chain fatty acids (LFCA) across the plasma membrane. SLC27A1 may act directly as a bona fide transporter, or alternatively, in a cytoplasmic or membrane-associated multimeric protein complex to trap and draw fatty acids towards accumulation. It also plays a pivotal role in regulating available LFCA substrates from exogenous sources in tissues undergoing high levels of beta-oxidation or triglyceride synthesis and may be involved in regulation of cholesterol metabolism. -
Cellular localization
Cell membrane; Single-pass membrane protein. Intracytoplasmic membrane; Single-pass membrane protein. Cytoplasm. Note=Plasma membrane and intracellular membranes, at least in adipocytes. Predominantly cytoplasmic in myocytes. -
Database links
- Entrez Gene: 376497 Human
- Omim: 600691 Human
- SwissProt: Q6PCB7 Human
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Alternative names
- ACSVL5 antibody
- FATP 1 antibody
- FATP antibody
see all
Images
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Western blot - Anti-FATP1 antibody (ab81875)All lanes : Anti-FATP1 antibody (ab81875) at 1 µg/ml
Lane 1 : Human skeletal muscle tissue lysate - total protein (ab29330)
Lane 2 : Human liver tissue lysate - total protein (ab29889)
Lane 3 : Human skeletal muscle tissue lysate - total protein (ab29330) with Immunising Peptide at 1 µg/ml
Lane 4 : Human liver tissue lysate - total protein (ab29889) with Immunising Peptide at 1 µg/ml
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 71 kDa
Observed band size: 65 kDa why is the actual band size different from the predicted?
Additional bands at: 35 kDa, 45 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 minute -
Immunocytochemistry/ Immunofluorescence - Anti-FATP1 antibody (ab81875)ICC/IF image of ab81875 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab81875 at 1µg/ml overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti- rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in This antibody also gave a positive result in Hek293, HepG2, and MCF-7 formaldehyde (4%, 10min) fixed cells at 1ug/ml, and also in HeLa, Hek293, HepG2, and MCF-7 Methanol (100%, 5min) fixed cells at 1ug/ml.
Protocols
Datasheets and documents
References (2)
ab81875 has been referenced in 2 publications.
- Sun Y et al. miR-142 controls metabolic reprogramming that regulates dendritic cell activation. J Clin Invest 129:2029-2042 (2019). PubMed: 30958798
- Li S et al. Betaine affects muscle lipid metabolism via regulating the fatty acid uptake and oxidation in finishing pig. J Anim Sci Biotechnol 8:72 (2017). WB ; Pig . PubMed: 28883917
Images
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Western blot - Anti-FATP1 antibody (ab81875)All lanes : Anti-FATP1 antibody (ab81875) at 1 µg/ml
Lane 1 : Human skeletal muscle tissue lysate - total protein (ab29330)
Lane 2 : Human liver tissue lysate - total protein (ab29889)
Lane 3 : Human skeletal muscle tissue lysate - total protein (ab29330) with Immunising Peptide at 1 µg/ml
Lane 4 : Human liver tissue lysate - total protein (ab29889) with Immunising Peptide at 1 µg/ml
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 71 kDa
Observed band size: 65 kDa why is the actual band size different from the predicted?
Additional bands at: 35 kDa, 45 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 minute
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Immunocytochemistry/ Immunofluorescence - Anti-FATP1 antibody (ab81875)
ICC/IF image of ab81875 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab81875 at 1µg/ml overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti- rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in This antibody also gave a positive result in Hek293, HepG2, and MCF-7 formaldehyde (4%, 10min) fixed cells at 1ug/ml, and also in HeLa, Hek293, HepG2, and MCF-7 Methanol (100%, 5min) fixed cells at 1ug/ml.
