Recombinant human Ubiquitin K48 only protein (ab157048)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: WB, Functional Studies, SDS-PAGE, MS
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Product name
Recombinant human Ubiquitin K48 only protein -
Biological activity
DUB reactivity: The potential utility of (K48-only) Ubn-ubiquitinylated conjugates as DUB substrates was demonstrated by their deconjugation in the presence of the isolated catalytic domain of USP2, a DUB of general and promiscuous activity that appears to exhibit no linkage preference. -
Purity
> 95 % SDS-PAGE.
High molecular weight conjugates were separated from free ubiquitin by size exclusion chromatography. -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human
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Preparation and Storage
- UBB
- UBB_HUMAN
- Ubiquitin
Images
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SDS-PAGE - Recombinant human Ubiquitin K48 only protein (ab157048)Coomassie stained SDS-PAGE gel showing ubiquitinylation of substrate protein (X) with (K48 only) Ub to give (K48 only) Ubn-ubiquitinylated conjugates (Z).
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Western blot - Recombinant human Ubiquitin K48 only protein (ab157048)Polyubiquitin-chain reactive
monoclonal antibody FK2 at 1/1000 dilution +Recombinant human Ubiquitin K48 only protein (ab157048) -
Western blot - Recombinant human Ubiquitin K48 only protein (ab157048)All lanes : PAb to ubiquitin-protein conjugates at 1/1000 dilution
Lane 1 : ab157048 incubated with USP2cd
Lane 2 : ab157048 incubated without USP2cd