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Cell Biology Proteolysis / Ubiquitin Proteasome / Ubiquitin Ubiquitin

Anti-HGS antibody (ab223753)

Anti-HGS antibody (ab223753)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to HGS
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-HGS antibody
    See all HGS primary antibodies
  • Description

    Rabbit polyclonal to HGS
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human HGS aa 300-450.
    Database link: O14964

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • WB: HEK-293T overexpressing HGS cell lysate. IHC-P: Human testis and cerebral cortex tissue. ICC/IF: U-2 OS cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Ubiquitin
    • Signal Transduction
    • Protein Phosphorylation
    • Tyrosine Kinases
    • Receptor Tyrosine Kinases

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HGS antibody (ab223753)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HGS antibody (ab223753)

    Paraffin-embedded human cerebral cortex tissue stained for HGS using ab223753 at 1/50 dilution in immunohistochemical analysis.

  • Western blot - Anti-HGS antibody (ab223753)
    Western blot - Anti-HGS antibody (ab223753)
    All lanes : Anti-HGS antibody (ab223753) at 1/100 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) vector only transfected cell lysate
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) overexpressing HGS (co-expressed with a C-terminal myc-DDK tag), cell lysate

    Developed using the ECL technique.

    Predicted band size: 86 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HGS antibody (ab223753)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HGS antibody (ab223753)

    Paraffin-embedded human testis tissue stained for HGS using ab223753 at 1/50 dilution in immunohistochemical analysis.

  • Immunocytochemistry/ Immunofluorescence - Anti-HGS antibody (ab223753)
    Immunocytochemistry/ Immunofluorescence - Anti-HGS antibody (ab223753)

    PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for HGS (green) using ab223753 at 4 µg/ml in ICC/IF.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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