Recombinant human NEDD4 protein (ab95927)
Key features and details
- Expression system: Baculovirus
- Active: Yes
- Tags: DDDDK tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
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Product name
Recombinant human NEDD4 protein -
Biological activity
Specific Activity: 0.0027 pmol/min/µg. Assay conditions: 50 µl reaction mix (4mM ATP, 5mM MgCl2, 1mM DTT, E1 (0.5 µg), E2 (2.8 µg), Biotin-Ubiquitin (0.5 µg) and NEDD4 (0-2 µg) in 50mM Tris pH 7.4) incubate for 30min at 37°C, then add to streptavidin coated plates and incubate for 30min at 37°C. Add antibody against NEDD4, incubate 1h. Finally, incubate 1h with the Europeum-labeled secondary antibody. Time resolved fluorescence measured at room temperature (Lambda exc=330 nm, Lambda em=620 nm). -
Purity
60 % .
Purity is >60% by SDS-PAGE. -
Expression system
Baculovirus -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Predicted molecular weight
86 kDa including tags -
Amino acids
150 to 900 -
Tags
DDDDK tag N-Terminus
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Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Store at -80°C.
pH: 8.00
Constituents: 0.0462% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.395% Tris HCl, 0.05% Tween, 20% Glycerol (glycerin, glycerine), 0.58% Sodium chloride
100 µg/ml FLAG peptideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
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Functional Studies - Recombinant human NEDD4 protein (ab95927)Image showing specific activity of ab95927.
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SDS-PAGE - Recombinant human NEDD4 protein (ab95927)10% SDS-PAGE analysis
Lane 1: ab95927 (4µg) Lane 2: Molecular weight markers