Antibodies, Proteins, Kits and Reagents for Life Science

311 587 ₸ Product size

100 µl 311 587 ₸

Order now and get it on Tuesday March 09, 2021

Anti-ACSL1 antibody [EPR13499] (ab177958)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR13499] to ACSL1
Suitable for: IHC-P, WB
Knockout validated
Reacts with: Human

Product name

Anti-ACSL1 antibody [EPR13499]
See all ACSL1 primary antibodies
Description

Rabbit monoclonal [EPR13499] to ACSL1
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human ACSL1 aa 650 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: P33121
Positive control
- HepG2, HeLa and Human fetal liver lysates; Human kidney and liver tissues.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IHC-P	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR13499
Isotype

IgG
Research areas

Western blot - Anti-ACSL1 antibody [EPR13499] (ab177958)

All lanes : Anti-ACSL1 antibody [EPR13499] (ab177958) at 1/1000 dilution

Lane 1 : Wild-type HeLa cell lysate
Lane 2 : ACSL1 knockout HeLa cell lysate
Lane 3 : HepG2 cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

Predicted band size: 78 kDa
Observed band size: 78 kDa

Lanes 1-3: Merged signal (red and green). Green - ab177958 observed at 78 kDa. Red - loading control ab8245 observed at 36 kDa.

ab177958 Anti-ACSL1 antibody [EPR13499] was shown to specifically react with ACSL1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265555 (knockout cell lysate ab257335) was used. Wild-type and ACSL1 knockout samples were subjected to SDS-PAGE. ab177958 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACSL1 antibody [EPR13499] (ab177958)

Immunohistochemical analysis of Human liver tissue labeling ACSL1 using ab177958 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACSL1 antibody [EPR13499] (ab177958)

Immunohistochemical analysis of Human kidney tissue labeling ACSL1 using ab177958 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Western blot - Anti-ACSL1 antibody [EPR13499] (ab177958)

All lanes : Anti-ACSL1 antibody [EPR13499] (ab177958) at 1/1000 dilution

Lane 1 : HepG2 cell lysate
Lane 2 : Human fetal liver lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 78 kDa
Anti-ACSL1 antibody [EPR13499] (ab177958)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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