Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ACSL1 antibody [EPR13499] (ab177958)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13499] to ACSL1
  • Suitable for: IHC-P, WB
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-ACSL1 antibody [EPR13499]
    See all ACSL1 primary antibodies

  • Description

    Rabbit monoclonal [EPR13499] to ACSL1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human ACSL1 aa 650 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: P33121

  • Positive control

    • HepG2, HeLa and Human fetal liver lysates; Human kidney and liver tissues.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-ACSL1 antibody [EPR13499] (ab177958)
    Western blot - Anti-ACSL1 antibody [EPR13499] (ab177958)
    All lanes : Anti-ACSL1 antibody [EPR13499] (ab177958) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : ACSL1 knockout HeLa cell lysate
    Lane 3 : HepG2 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 78 kDa
    Observed band size: 78 kDa



    Lanes 1-3: Merged signal (red and green). Green - ab177958 observed at 78 kDa. Red - loading control ab8245 observed at 36 kDa.

    ab177958 Anti-ACSL1 antibody [EPR13499] was shown to specifically react with ACSL1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265555 (knockout cell lysate ab257335) was used. Wild-type and ACSL1 knockout samples were subjected to SDS-PAGE. ab177958 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACSL1 antibody [EPR13499] (ab177958)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACSL1 antibody [EPR13499] (ab177958)

    Immunohistochemical analysis of Human liver tissue labeling ACSL1 using ab177958 at 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACSL1 antibody [EPR13499] (ab177958)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACSL1 antibody [EPR13499] (ab177958)

    Immunohistochemical analysis of Human kidney tissue labeling ACSL1 using ab177958 at 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-ACSL1 antibody [EPR13499] (ab177958)
    Western blot - Anti-ACSL1 antibody [EPR13499] (ab177958)
    All lanes : Anti-ACSL1 antibody [EPR13499] (ab177958) at 1/1000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : Human fetal liver lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 78 kDa

  • Anti-ACSL1 antibody [EPR13499] (ab177958)
    Anti-ACSL1 antibody [EPR13499] (ab177958)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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