Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸ 10 µl 120 614 ₸

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Anti-ACSL1 antibody [EPR13498] (ab178419)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR13498] to ACSL1
Suitable for: IHC-P, WB
Knockout validated
Reacts with: Human

Product name

Anti-ACSL1 antibody [EPR13498]
See all ACSL1 primary antibodies
Description

Rabbit monoclonal [EPR13498] to ACSL1
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide within Human ACSL1 aa 100-200 (Cysteine residue). The exact sequence is proprietary.
Database link: P33121
Positive control
- Human fetal liver, heart and kidney lysates; HeLa and HepG2 cell lysates; Human heart, kidney and liver tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IHC-P	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR13498
Isotype

IgG
Research areas

Western blot - Anti-ACSL1 antibody [EPR13498] (ab178419)

All lanes : Anti-ACSL1 antibody [EPR13498] (ab178419) at 1/1000 dilution

Lane 1 : Wild-type HeLa cell lysate
Lane 2 : ACSL1 knockout HeLa cell lysate
Lane 3 : HepG2 cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

Predicted band size: 78 kDa
Observed band size: 78 kDa

Lanes 1-3: Merged signal (red and green). Green - ab178419 observed at 78 kDa. Red - loading control ab8245 observed at 36 kDa.

ab178419 Anti-ACSL1 antibody [EPR13498] was shown to specifically react with ACSL1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265555 (knockout cell lysate ab257335) was used. Wild-type and ACSL1 knockout samples were subjected to SDS-PAGE. ab178419 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACSL1 antibody [EPR13498] (ab178419)

Immunohistochemical analysis of paraffin-embedded Human heart tissue labeling ACSL1 with ab178419 at 1/50 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Western blot - Anti-ACSL1 antibody [EPR13498] (ab178419)

All lanes : Anti-ACSL1 antibody [EPR13498] (ab178419) at 1/1000 dilution

Lane 1 : Human fetal liver lysate
Lane 2 : Human fetal heart lysate
Lane 3 : Human fetal kidney lysate
Lane 4 : HepG2 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 78 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACSL1 antibody [EPR13498] (ab178419)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling ACSL1 with ab178419 at 1/50 dilution

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACSL1 antibody [EPR13498] (ab178419)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling ACSL1 with ab178419 at 1/50 dilution

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Anti-ACSL1 antibody [EPR13498] (ab178419)

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