Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human prostate carcinoma tissue sections labeling KRT6C with ab227643 at 1/200 dilution (0.12 μg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Leica DS9800 (Bond™ Polymer Refine Detection) was used as a secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the human prostate carcinoma, performed on a Leica Biosystems BOND™ RX instrument. 
The section was incubated with ab227643 for 10 mins at room temperature.

Immunocytochemistry/ Immunofluorescence analysis of HaCaT (human skin keratinocyte) cells labeling KRT6C with purified ab227643 at 1/50 (1.92 µg/ml). Cells were fixed in 100% Methanol. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

Flow cytometry analysis of HaCaT (human skin keratinocyte) labeling KRT6C with purified ab227643 at 1/200 dilution (0.48 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotype control - Rabbit monoclonal IgG (ab172730) (black). Unlableled control - Unlabelled cells (blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat skin tissue sections labeling KRT6C with ab227643 at 1/200 dilution (0.12 μg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Leica DS9800 (Bond™ Polymer Refine Detection) was used as a secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the rat skin, performed on a Leica Biosystems BOND™ RX instrument. 
The section was incubated with ab227643 for 10 mins at room temperature.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse skin tissue sections labeling KRT6C with ab227643 at 1/200 dilution (0.12 μg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Leica DS9800 (Bond™ Polymer Refine Detection) was used as a secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the mouse skin, performed on a Leica Biosystems BOND™ RX instrument. 
The section was incubated with ab227643 for 10 mins at room temperature.

Anti-KRT6C antibody [SP87] (ab227643) at 1/25 dilution + A431 (human epidermoid carcinoma cell line) whole cell lysate

Developed using the ECL technique.

Predicted band size: 60 kDa

Formalin-fixed, paraffin-embedded human prostate carcinoma tissue stained for KRT6C using ab227643 at 1/200 dilution in immunohistochemical analysis.

Immunohistochemistry (Frozen) analysis of rat skin tissue section labeling KRT6C with purified ab227643 at 1/25 (3.84µg/ml). Sections were fixed in 4% paraformaldehyde and permeabilized with 0.2% Triton X-100. Antigen retrieval was Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

Immunohistochemistry (Frozen) analysis of mouse skin tissue section labeling KRT6C with purified ab227643 at 1/25 (3.84µg/ml). Sections were fixed in 4% paraformaldehyde and permeabilized with 0.2% Triton X-100. Antigen retrieval was Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

Flow cytometric analysis of A431 (human epidermoid carcinoma cell line) cell line labeling KRT6C with ab227643 at 1/100 dilution (green) compared with a negative control rabbit IgG (blue). 

Formalin-fixed, paraffin-embedded human breast tissue stained for KRT6C using ab227643 at 1/200 dilution in immunohistochemical analysis.

Formalin-fixed, paraffin-embedded human stomach tissue stained for KRT6C using ab227643 at 1/200 dilution in immunohistochemical analysis.

Formalin-fixed, paraffin-embedded human prostate tissue stained for KRT6C using ab227643 at 1/200 dilution in immunohistochemical analysis.

Formalin-fixed, paraffin-embedded human lung squamous cell carcinoma tissue stained for KRT6C using ab227643 at 1/200 dilution in immunohistochemical analysis.

Formalin-fixed, paraffin-embedded human rectal adenocarcinoma tissue stained for KRT6C using ab227643 at 1/200 dilution in immunohistochemical analysis.

Formalin-fixed, paraffin-embedded human bladder tissue stained for KRT6C using ab227643 at 1/200 dilution in immunohistochemical analysis.

Formalin-fixed, paraffin-embedded human bladder transitional cell carcinoma tissue stained for KRT6C using ab227643 at 1/200 dilution in immunohistochemical analysis.

Formalin-fixed, paraffin-embedded human colon adenocarcinoma tissue stained for KRT6C using ab227643 at 1/200 dilution in immunohistochemical analysis.

Formalin-fixed, paraffin-embedded human ovarian adenocarcinoma tissue stained for KRT6C using ab227643 at 1/200 dilution in immunohistochemical analysis.

Formalin-fixed, paraffin-embedded human placenta tissue stained for KRT6C using ab227643 at 1/200 dilution in immunohistochemical analysis.