Anti-KRT6C antibody [SP87] (ab227643)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP87] to KRT6C
- Suitable for: IHC-P, WB, Flow Cyt, IHC-Fr, ICC/IF
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-KRT6C antibody [SP87]
See all KRT6C primary antibodies -
Description
Rabbit monoclonal [SP87] to KRT6C -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanIHC-Fr MouseRatIHC-P MouseRatHumanWB Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: A431 whole cell lysate. IHC-P: Human prostate carcinoma, breast, stomach, prostate, lung squamous cell carcinoma, rectal adenocarcinoma, bladder, bladder transitional cell carcinoma, colon adenocarcinoma, and placenta tissue, rat and mouse skin Flow Cyt: A431 and HaCaT cells. ICC/IF: HaCaT cells. IHC-Fr: Rat and mouse skin tissue.
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General notes
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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Purity
Protein A/G purified -
Purification notes
Purified from TCS by protein A/G. -
Clonality
Monoclonal -
Clone number
SP87 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human prostate carcinoma tissue sections labeling KRT6C with ab227643 at 1/200 dilution (0.12 μg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Leica DS9800 (Bond™ Polymer Refine Detection) was used as a secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the human prostate carcinoma, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab227643 for 10 mins at room temperature. -
Immunocytochemistry/ Immunofluorescence analysis of HaCaT (human skin keratinocyte) cells labeling KRT6C with purified ab227643 at 1/50 (1.92 µg/ml). Cells were fixed in 100% Methanol. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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Flow cytometry analysis of HaCaT (human skin keratinocyte) labeling KRT6C with purified ab227643 at 1/200 dilution (0.48 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotype control - Rabbit monoclonal IgG (ab172730) (black). Unlableled control - Unlabelled cells (blue).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat skin tissue sections labeling KRT6C with ab227643 at 1/200 dilution (0.12 μg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Leica DS9800 (Bond™ Polymer Refine Detection) was used as a secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the rat skin, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab227643 for 10 mins at room temperature. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse skin tissue sections labeling KRT6C with ab227643 at 1/200 dilution (0.12 μg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Leica DS9800 (Bond™ Polymer Refine Detection) was used as a secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the mouse skin, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab227643 for 10 mins at room temperature. -
Anti-KRT6C antibody [SP87] (ab227643) at 1/25 dilution + A431 (human epidermoid carcinoma cell line) whole cell lysate
Developed using the ECL technique.
Predicted band size: 60 kDa
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Formalin-fixed, paraffin-embedded human prostate carcinoma tissue stained for KRT6C using ab227643 at 1/200 dilution in immunohistochemical analysis.
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Immunohistochemistry (Frozen) analysis of rat skin tissue section labeling KRT6C with purified ab227643 at 1/25 (3.84µg/ml). Sections were fixed in 4% paraformaldehyde and permeabilized with 0.2% Triton X-100. Antigen retrieval was Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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Immunohistochemistry (Frozen) analysis of mouse skin tissue section labeling KRT6C with purified ab227643 at 1/25 (3.84µg/ml). Sections were fixed in 4% paraformaldehyde and permeabilized with 0.2% Triton X-100. Antigen retrieval was Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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Flow cytometric analysis of A431 (human epidermoid carcinoma cell line) cell line labeling KRT6C with ab227643 at 1/100 dilution (green) compared with a negative control rabbit IgG (blue).
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Formalin-fixed, paraffin-embedded human breast tissue stained for KRT6C using ab227643 at 1/200 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human stomach tissue stained for KRT6C using ab227643 at 1/200 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human prostate tissue stained for KRT6C using ab227643 at 1/200 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human lung squamous cell carcinoma tissue stained for KRT6C using ab227643 at 1/200 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human rectal adenocarcinoma tissue stained for KRT6C using ab227643 at 1/200 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human bladder tissue stained for KRT6C using ab227643 at 1/200 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human bladder transitional cell carcinoma tissue stained for KRT6C using ab227643 at 1/200 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human colon adenocarcinoma tissue stained for KRT6C using ab227643 at 1/200 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human ovarian adenocarcinoma tissue stained for KRT6C using ab227643 at 1/200 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human placenta tissue stained for KRT6C using ab227643 at 1/200 dilution in immunohistochemical analysis.
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