Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-VISTA antibody [EPR21050] - BSA and Azide free (ab238886)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR21050] to VISTA - BSA and Azide free
  • Suitable for: WB, IHC-P, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-VISTA antibody [EPR21050] - BSA and Azide free
    See all VISTA primary antibodies

  • Description

    Rabbit monoclonal [EPR21050] to VISTA - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, IPmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human spleen tissue.

  • General notes

    Ab238886 is the carrier-free version of ab230950. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab238886 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR21050

  • Isotype

    IgG

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VISTA antibody [EPR21050] - BSA and Azide free (ab238886)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VISTA antibody [EPR21050] - BSA and Azide free (ab238886)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue labeling VISTA with ab230950 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP) secondary antibody. Membranous and cytoplasmic staining in tumor-in?ltrating leukocytes of human lung cancer (PMID: 28236118, PMID: 28507801) is observed. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab230950).

  • Immunoprecipitation - Anti-VISTA antibody [EPR21050] - BSA and Azide free (ab238886)
    Immunoprecipitation - Anti-VISTA antibody [EPR21050] - BSA and Azide free (ab238886)

    VISTA was immunoprecipitated from 0.35 mg DU145 (Human prostate carcinoma epithelial cell) whole cell lysate with ab230950 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab230950 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.
    Lane 1: DU154 whole cell lysate 10 µg (Input).
    Lane 2: ab230950 IP in DU154 whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab230950 in DU154 whole cell lysate.
    Blocking/Dilution buffer: 5% NFDM/TBST.
    Exposure time: 3 minutes.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab230950).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VISTA antibody [EPR21050] - BSA and Azide free (ab238886)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VISTA antibody [EPR21050] - BSA and Azide free (ab238886)

    Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling VISTA with ab230950 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP) secondary antibody. Membranous and cytoplasmic staining in leukocytes of human spleen (PMID: 21383057, PMID: 24691993) is observed. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab230950).

  • Anti-VISTA antibody [EPR21050] - BSA and Azide free (ab238886)
    Anti-VISTA antibody [EPR21050] - BSA and Azide free (ab238886)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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